A total number of 50 apparently healthy, adult buffaloes of both the sexes were examined for different physical and biochemical parameters of synovial fluid from the tibio-tarsal joints. The physical and biochemical parameters which was studied during present investigation were as follows: clarity, colour, specific gravity, pH, viscosity, spontaneous clot formation and total volume (ml), ALT (IU/L), glucose (mg/dl), total protein(g/dl) and bilirubin (mg/dl). Out of the 50 samples, 30% of the samples were colorless clear whereas 70% of the samples found were slightly yellow in colour and 90% of the samples had clear transparency whereas 10% were showed slight turbidity. The material was collected from buffaloes brought in the Cantonment Board slaughter house, Mhow (M.P.).
