The simultaneous determination of phenylephrine hydrochloride, paracetamol, chlorpheniramine maleate and dextromethorphan hydrobromide in pharmaceutical preparations

被引：31

作者：

Palabiyik, I. M. ^{[1
]}

Onur, F. ^{[1
]}

机构：

[1] Hacettepe Univ, Fac Pharm, Dept Analyt Chem, TR-06100 Ankara, Turkey

来源：

CHROMATOGRAPHIA | 2007年 / 66卷 / Suppl 1期

关键词：

column liquid chromatography; dextromethorphan hydrobromide; phenylephrine hydrochloride; paracetamol; chlorpheniramine maleate;

D O I：

10.1365/s10337-007-0324-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new rapid and sensitive method has been developed and validated for the simultaneous determination of phenylephrine hydrochloride, paracetamol, chlorpheniramine maleate and clextromethorphan hydrobromide in pharmaceutical preparations. The separation was achieved on a C18 column using a gradient mobile phase of acetonitrile-soclium perchlorate (pH 3, 0.01 M) at a flow rate of 1.4 mL min(-1). Detection was at 204 nm. Pseudoephedrine hydrochloride was selected as internal standard. The recovery of the drugs ranged from 97.8 to 100.9%. Central composite design was used during validation to calculate method robustness and the percentage of sodium perchlorate, temperature and flow rate were investigated as factors. The method was found to be applicable for the determination of the four compounds in sugar-coated tablets.

引用

页码：S93 / S96

页数：4

共 16 条

[1] Simultaneous quantitation of five active principles in a pharmaceutical preparation:: Development and validation of a near infrared spectroscopic method [J].

Blanco, M ;

Alcalá, M .

EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2006, 27 (2-3) :280-286

[2] Simultaneous determination of paracetamol and chlorpheniramine in human plasma by liquid chromatography-tandem mass spectrometry [J].

Celma, C ;

Allué, JA ;

Pruñonosa, J ;

Peraire, C ;

Obach, R .

JOURNAL OF CHROMATOGRAPHY A, 2000, 870 (1-2) :77-86

[3] Simultaneous determination of triprolidine, pseudoephedrine, paracetamol and dextromethorphan by HPLC [J].

DeOrsi, D ;

Gagliardi, L ;

Bolasco, A ;

Tonelli, D .

CHROMATOGRAPHIA, 1996, 43 (9-10) :496-500

[4] Quantitative analysis of chlorpheniramine maleate and phenylephrine hydrochloride in nasal drops by differential-derivative spectrophotometric, zero-crossing first derivative UV spectrophotometric and absorbance ratio methods [J].

Erk, N .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2000, 23 (06) :1023-1031

[5] Simultaneous high performance liquid chromatographic and derivative ratio spectra spectrophotometry determination of chlorpheniramine maleate and phenylephrine hydrochloride [J].

Erk, N ;

Kartal, M .

FARMACO, 1998, 53 (8-9) :617-622

[6] Robustness testing in liquid chromatography and capillary electrophoresis [J].

Fabre, H .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1996, 14 (8-10) :1125-1132

[7] Poly(ethyleneglycol) column for the determination of acetaminophen, phenylephrine and chlorpheniramine in pharmaceutical formulations [J].

García, A ;

Rupérez, FJ ;

Marín, A ;

de la Maza, A ;

Barbas, C .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2003, 785 (02) :237-243

[8]

HUANG X, 2003, ZHONGGUO LINCHUANG Y, V12, P223

[9]

ICH, 2005, ICH HARMONIZED TRIPA

[10] CE versus HPLC for the dissolution test in a pharmaceutical formulation containing acetaminophen, phenylephrine and chlorpheniramine [J].

Marín, A ;

Barbas, C .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2004, 35 (04) :769-777

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