Local protein synthesis mediates a rapid increase in dendritic elongation factor 1A after induction of late long-term potentiation

被引:192
作者
Tsokas, P
Grace, EA
Chan, P
Ma, T
Sealfon, SC
Iyengar, R
Landau, EM
Blitzer, RD
机构
[1] Mt Sinai Sch Med, Dept Pharmacol & Biol Chem, New York, NY 10029 USA
[2] Mt Sinai Sch Med, Dept Neurosci, New York, NY 10029 USA
[3] Mt Sinai Sch Med, Dept Psychiat, New York, NY 10029 USA
[4] Bronx Vet Affairs Med Ctr, Psychiat Serv, Bronx, NY 10468 USA
关键词
hippocampus; LTP; local protein synthesis; mTOR; eEF1A; TOP mRNA;
D O I
10.1523/JNEUROSCI.0599-05.2005
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The maintenance of long-term potentiation (LTP) requires a brief period of accelerated protein synthesis soon after synaptic stimulation, suggesting that an early phase of enhanced translation contributes to stable LTP. The mechanism regulating protein synthesis and the location and identities of mRNAs translated are not well understood. Here, we show in acute brain slices that the induction of protein synthesis-dependent hippocampal LTP increases the expression of elongation factor 1A (eEF1A), the mRNA of which contains a 5' terminal oligopyrimidine tract. This effect is blocked by rapamycin, indicating that the increase in EF1A expression is mediated by the mammalian target of rapamycin ( mTOR) pathway. We find that mRNA for eEF1A is present in pyramidal cell dendrites and that the LTP-associated increase in eEF1A expression was intact in dendrites that had been severed from their cell bodies before stimulation. eEF1A levels increased within 5 min after stimulation in a translation-dependent manner, and this effect remained stable for 3 h. These results suggest a mechanism whereby synaptic stimulation, by signaling through the mTOR pathway, produces an increase in dendritic translational capacity that contributes to LTP maintenance.
引用
收藏
页码:5833 / 5843
页数:11
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