Proteome-Wide Survey of Cysteine Oxidation by Using a Norbornene Probe

被引：14

作者：

Alcock, Lisa J. ^{[1
,2
]}

Langini, Maike ^{[3
,4
,5
,6
,7
]}

Stuehler, Kai ^{[3
,8
]}

Remke, Marc ^{[4
,5
,6
,7
]}

Perkins, Michael, V ^{[1
]}

Bernardes, Goncalo J. L. ^{[9
,10
]}

Chalker, Justin M. ^{[1
,2
]}

机构：

[1] Flinders Univ S Australia, Coll Sci & Engn, Sturt Rd, Bedford Pk, SA 5042, Australia

[2] Flinders Univ S Australia, Inst Nanoscale Sci & Technol, Sturt Rd, Bedford Pk, SA 5042, Australia

[3] Heinrich Heine Univ, Biomed Res Ctr BMFZ, MPL, Univ Str 1, D-40225 Dusseldorf, Germany

[4] German Canc Res Ctr, Div Pediat Neurooncogen, Moorenstr 5, D-40225 Dusseldorf, Germany

[5] German Canc Consortium DKTK, Partner Site Univ Hosp Dusseldorf, Moorenstr 5, D-40225 Dusseldorf, Germany

[6] Heinrich Heine Univ, Med Fac, Dept Pediat Oncol Hematol & Clin Immunol, Univ Str 1, D-40225 Dusseldorf, Germany

[7] Heinrich Heine Univ Dusseldorf, Med Fac, Inst Neuropathol, Moorenstr 5, D-40225 Dusseldorf, Germany

[8] Univ Hosp Dusseldorf, Inst Mol Med, Univ Str 1, D-40225 Dusseldorf, Germany

[9] Univ Cambridge, Dept Chem, Lensfield Rd, Cambridge CB2 1EW, England

[10] Univ Lisbon, Fac Med, Inst Med Mol, Ave Prof Egas Moniz, P-1649028 Lisbon, Portugal

来源：

CHEMBIOCHEM | 2020年 / 21卷 / 09期

基金：

澳大利亚研究理事会;

关键词：

cysteine oxidation; norbornene; oxidative stress; sulfenic acid; sulfenome; SULFENIC ACID DETECTION; SIGNAL-TRANSDUCTION; MODIFIED PROTEINS; CHEMICAL APPROACH; STRESS; DISEASE; IDENTIFICATION; REACTIVITY; DIMEDONE;

D O I：

10.1002/cbic.201900729

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rapid detection of cysteine oxidation in living cells is critical in advancing our understanding of responses to reactive oxygen species (ROS) and oxidative stress. Accordingly, there is a need to develop chemical probes that facilitate proteome-wide detection of cysteine's many oxidation states. Herein, we report the first whole-cell proteomics analysis using a norbornene probe to detect the initial product of cysteine oxidation: cysteine sulfenic acid. The oxidised proteins identified in the HeLa cell model represent the first targets of the ROS hydrogen peroxide. The panel of protein hits provides new and important information about the targets of oxidative stress, including 148 new protein members of the sulfenome. These findings provide new leads for the study and understanding of redox signalling and diseases associated with oxidative stress.

引用

页码：1329 / 1334

页数：6

共 42 条

[1] Chemical proteomics reveals new targets of cysteine sulfinic acid reductase [J].