Phage-displayed peptides that mimic zearalenone and its application in immunoassay

被引：58

作者：

He, Qing-Hua ^{[1
]}

Xu, Yang ^{[1
]}

Huang, Yun-Hong ^{[1
,2
]}

Liu, Ren-Rong ^{[3
]}

Huang, Zhi-Bing ^{[1
]}

Li, Yan-Ping ^{[1
]}

机构：

[1] Nanchang Univ, Sino Germany Joint Res Inst, State Key Lab Food Sci & Technol, Nanchang 330047, Peoples R China

[2] Jiangxi Normal Univ, Sch Life Sci, Nanchang 330022, Peoples R China

[3] Jiangxi Sci & Technol Normal Univ, Sch Life Sci, Nanchang 330047, Peoples R China

来源：

FOOD CHEMISTRY | 2011年 / 126卷 / 03期

基金：

国家高技术研究发展计划(863计划);

关键词：

Zearalenone; Phage-displayed peptide; Immunoassay; LINKED-IMMUNOSORBENT-ASSAY; ALPHA-ZEARALENOL; OCHRATOXIN-A; ANTIBODY; CELLS; CHROMATOGRAPHY; VERO; FEED;

D O I：

10.1016/j.foodchem.2010.11.085

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Monoclonal antibody 7G5 (McAb 7G5), which recognises the zearalenone (ZEN), was used to select for peptides that mimic the ZEN by employing a library of filamentous phages that have 7-mer peptides on their surfaces. After three rounds of panning, two mimotope peptides were obtained to be able to mimic ZEN in binding with the McAb 7G5, their amino acid sequences were DAVILLM and HHCHWWH. Enzyme-linked immunosorbent assay (ELISA) for detect ZEN was established with the phages. In the most sensitive assay, the linear range of the inhibition curve was 100-10,000 pg/ml, the detection limit was 100 pg/ml. The results demonstrated that those phage peptides could be used as the surrogate of ZEN to establish the immunoassay. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：1312 / 1315

页数：4