Enhanced tolerance to heat stress in transgenic plants expressing the GASA4 gene

被引：66
作者：
Ko, Chang-Beom
Woo, Young-Min
Lee, Dong Ju
Lee, Myung-Chul
Kim, Cheol Soo
机构：
[1] Chonnam Natl Univ, Agr Plant Stress Res Ctr, Dept Plant Biotechnol, Kwangju 500757, South Korea
[2] Pohang Univ Sci & Technol, Div Mol & Life Sci, Pohang 790784, South Korea
[3] Natl Inst Agr Biotechnol, Suwon 441707, South Korea
来源：
PLANT PHYSIOLOGY AND BIOCHEMISTRY | 2007年 / 45卷 / 09期
关键词：
BiP; chaperone; GASA4; heat stress; thermo-tolerance; yeast screening;
D O I：
10.1016/j.plaphy.2007.07.010
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
We conducted a genetic yeast screen to identify Thermo-tolerance genes (TTOs) in maize kernel cDNA library. During the screening, we identified a maize clone (TTO6) that seemed to confer elevated heat tolerance in comparison to control cells. TTO6 cDNA (GenBank accession no. AY103785) encodes an 11-kDa protein which is 69% similarity to the Arabidopsis GASA4 gene. To further examine heat tolerance in Arabidopsis, we functionally characterized the GASA4 gene and found that heat induced GASA4 expression. Constitutive expression of GASA4 in Arabidopsis led to elevated heat tolerance in transgenic lines. Interestingly, endoplasmic reticulum chaperone expression analysis suggests that GASA4 influences BiP gene expression during heat stress. (C) 2007 Elsevier Masson SAS. All rights reserved.
引用
收藏
页码：722 / 728
页数：7
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