Expression of MMP-8 and MMP-13 genes in the periodontal ligament during tooth movement in rats

被引：79
作者：
Takahashi, I
Nishimura, M
Onodera, K
Bae, JW
Mitani, H
Okazaki, M
Sasano, Y
Mitani, H
机构：
[1] Tohoku Univ, Grad Sch Dent, Div Orthodont & Dentofacial Orthoped, Aoba Ku, Sendai, Miyagi 9808575, Japan
[2] Tohoku Univ, Grad Sch Dent, Div Mol Biol, Aoba Ku, Sendai, Miyagi 9808575, Japan
来源：
JOURNAL OF DENTAL RESEARCH | 2003年 / 82卷 / 08期
关键词：
remodeling; collagen; collagenase; periodontal ligament;
D O I：
10.1177/154405910308200815
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
Periodontal ligament tissue is remodeled on both the tension and compression sides of moving teeth during orthodontic tooth movement. The present study was designed to clarify the hypothesis that the expression of MMP-8 and MMP-13 mRNA is promoted during the remodeling of periodontal ligament tissue in orthodontic tooth movement. We used the in situ hybridization method and semi-quantitative reverse-transcription/polymerase chain-reaction analysis to elucidate the gene expression of MMP-8 and MMP-13 mRNA. Expression of MMP-8 and MMP-13 mRNA transiently increased on both the compression and tension sides during active tooth movement in vivo. The gene expression of MMP-8 and MMP-13 was induced by tension, while compression indirectly promoted the gene expression of MMP-8 and MMP-13 through soluble factors in vitro. Thus, we concluded that the expression of MMP-8 and MMP-13 is differentially regulated by tension and compression, and plays an important role in the remodeling of the periodontal ligament.
引用
收藏
页码：646 / 651
页数：6
相关论文
共 29 条
  • [21] Inverse dose- and time-dependent effect of basic fibroblast growth factor on the gene expression of collagen type I and matrix metalloproteinase-1 by periodontal ligament cells in culture
    Palmon, A
    Roos, H
    Edel, J
    Zax, B
    Savion, N
    Grosskop, A
    Pitaru, S
    [J]. JOURNAL OF PERIODONTOLOGY, 2000, 71 (06) : 974 - 980
  • [22] Inhibition of matrix metalloproteinase-mediated periodontal bone loss in rats: A comparison of 6 chemically modified tetracyclines
    Ramamurthy, NS
    Rifkin, BR
    Greenwald, RA
    Xu, JW
    Liu, Y
    Turner, G
    Golub, LM
    Vernillo, AT
    [J]. JOURNAL OF PERIODONTOLOGY, 2002, 73 (07) : 726 - 734
  • [23] The effect of mechanical force on mRNA levels of collagenase, collagen type 1, and tissue inhibitors of metalloproteinases in Gingivae of dogs
    Redlich, M
    Reichenberg, E
    Harari, D
    Zaks, B
    Shoshan, S
    Palmon, A
    [J]. JOURNAL OF DENTAL RESEARCH, 2001, 80 (12) : 2080 - 2084
  • [24] RYGH P, 1989, BIOL TOOTH MOVEMENT, P9
  • [25] How matrix metalloproteinases regulate cell behavior
    Sternlicht, MD
    Werb, Z
    [J]. ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 2001, 17 : 463 - 516
  • [26] Effects of expansive force on the differentiation of midpalatal suture cartilage in rats
    Takahashi, I
    Mizoguchi, I
    Nakamura, M
    Sasano, Y
    Saitoh, S
    Kagayama, M
    Mitani, H
    [J]. BONE, 1996, 18 (04) : 341 - 348
  • [27] Expression of MMP-8 and MMP-13 mRNAs in rat periodontium during tooth eruption
    Tsubota, M
    Sasano, Y
    Takahashi, I
    Kagayama, M
    Shimauchi, H
    [J]. JOURNAL OF DENTAL RESEARCH, 2002, 81 (10) : 673 - 678
  • [28] Phagocytosis of fibronectin and collagens type I, III, and V by human gingival and periodontal ligament fibroblasts in vitro
    van der Pauw, MT
    Van den Bos, T
    Everts, V
    Beertsen, W
    [J]. JOURNAL OF PERIODONTOLOGY, 2001, 72 (10) : 1340 - 1347
  • [29] Production of matrix-degrading enzymes and inhibition of osteoclast-like cell differentiation by fibroblast-like cells from the periodontal ligament of human primary teeth
    Wu, YM
    Richards, DW
    Rowe, DJ
    [J]. JOURNAL OF DENTAL RESEARCH, 1999, 78 (02) : 681 - 689
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