Novel cell surface luciferase reporter for high-throughput yeast one-hybrid screens

被引：11

作者：

Bonaldi, Katia ^{[1
,2
]}

Li, Zheng ^{[1
,2
]}

Kang, S. Earl ^{[1
,4
]}

Breton, Ghislain ^{[3
]}

Pruneda-Paz, Jose L. ^{[1
,2
]}

机构：

[1] Univ Calif San Diego, Div Biol Sci, La Jolla, CA 92093 USA

[2] Univ Calif San Diego, Ctr Circadian Biol, La Jolla, CA 92093 USA

[3] McGovern Med Sch, Dept Integrat Biol & Pharmacol, Houston, TX 77030 USA

[4] Univ Georgia, Dept Plant Biol, Athens, GA 30602 USA

来源：

NUCLEIC ACIDS RESEARCH | 2017年 / 45卷 / 18期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

GENE REGULATORY NETWORK; TRANSCRIPTION FACTORS; CIRCADIAN CLOCK; SACCHAROMYCES-CEREVISIAE; FUNCTIONAL GENOMICS; BETA-GALACTOSIDASE; ARABIDOPSIS; ASSAYS; EXPRESSION; SYSTEM;

D O I：

10.1093/nar/gkx682

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Gene-centered yeast one-hybrid (Y1H) screens provide a powerful and effective strategy to identify transcription factor (TF)-promoter interactions. While genome-wide TF ORFeome clone collections are increasingly available, screening protocols have limitations inherent to the properties of the enzymatic reaction used to identify interactions and to the procedure required to perform the assay in a high-throughput format. Here, we present the development and validation of a streamlined strategy for quantitative and fully automated gene-centered Y1H screens using a novel cell surface Gaussia luciferase reporter.

引用

页数：12

共 38 条

[1] Human Gene-Centered Transcription Factor Networks for Enhancers and Disease Variants
Bass, Juan I. Fuxman
Sahni, Nidhi
Shrestha, Shaleen
Garcia-Gonzalez, Aurian
Mori, Akihiro
Bhat, Numana
Yi, Song
Hill, David E.
Vidal, Marc
Walhout, Albertha J. M.
[J]. CELL, 2015, 161 (03) : 661 - 673
[2] Breton G, 2016, METHODS MOL BIOL, V1398, P107, DOI 10.1007/978-1-4939-3356-3_10
[3] The Maize TFome - development of a transcription factor open reading frame collection for functional genomics
Burdo, Brett
Gray, John
Goetting-Minesky, Mary P.
Wittler, Bettina
Hunt, Matthew
Li, Tai
Velliquette, David
Thomas, Julie
Gentzel, Irene
Brito, Michael dos Santos
Mejia-Guerra, Maria Katherine
Connolly, Layne N.
Qaisi, Dalya
Li, Wei
Casas, Maria I.
Doseff, Andrea I.
Grotewold, Erich
[J]. PLANT JOURNAL, 2014, 80 (02) : 356 - 366
[4] Transcriptional Regulation of LUX by CBF1 Mediates Cold Input to the Circadian Clock in Arabidopsis
Chow, Brenda Y.
Sanchez, Sabrina E.
Breton, Ghislain
Pruneda-Paz, Jose L.
Krogan, Naden T.
Kay, Steve A.
[J]. CURRENT BIOLOGY, 2014, 24 (13) : 1518 - 1524
[5] Real-time RT-PCR profiling of over 1400 Arabidopsis transcription factors:: unprecedented sensitivity reveals novel root- and shoot-specific genes
Czechowski, T
Bari, RP
Stitt, M
Scheible, WR
Udvardi, MK
[J]. PLANT JOURNAL, 2004, 38 (02) : 366 - 379
[6] TCP Transcription Factors at the Interface between Environmental Challenges and the Plant's Growth Responses
Danisman, Selahattin
[J]. FRONTIERS IN PLANT SCIENCE, 2016, 7
[7] A gateway-compatible yeast one-hybrid system
Deplancke, B
Dupuy, D
Vidal, M
Walhout, AJM
[J]. GENOME RESEARCH, 2004, 14 (10B) : 2093 - 2101
[8] A gene-centered C. elegans protein-DNA interaction network
Deplancke, Bart
Mukhopadhyay, Arnab
Ao, Wanyuan
Elewa, Ahmed M.
Grove, Christian A.
Martinez, Natalia J.
Sequerra, Reynaldo
Doucette-Stamm, Lynn
Reece-Hoyes, John S.
Hope, Ian A.
Tissenbaum, Heidi A.
Mango, Susan E.
Walhout, Albertha J. M.
[J]. CELL, 2006, 125 (06) : 1193 - 1205
[9] A Multifunctional, Synthetic Gaussia princeps Luciferase Reporter for Live Imaging of Candida albicans Infections
Enjalbert, Brice
Rachini, Anna
Vediyappan, Govindsamy
Pietrella, Donatella
Spaccapelo, Roberta
Vecchiarelli, Anna
Brown, Alistair J. P.
d'Enfert, Christophe
[J]. INFECTION AND IMMUNITY, 2009, 77 (11) : 4847 - 4858
[10] Gaudinier A, 2011, NAT METHODS, V8, P1053, DOI [10.1038/NMETH.1750, 10.1038/nmeth.1750]

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