Aquaporin subtypes in rat cerebral microvessels

被引：65

作者：

Kobayashi, H

Minami, S

Itoh, S

Shiraishi, S

Yokoo, H

Yanagita, T

Uezono, Y

Mohri, M

Wada, A

机构：

[1] Miyazaki Med Coll, Dept Pharmacol, Miyazaki 891692, Japan

[2] Japan Marine Sci & Technol Ctr, Marine Ecosyst Res Dept, Yokosuka, Kanagawa 2370061, Japan

来源：

NEUROSCIENCE LETTERS | 2001年 / 297卷 / 03期

关键词：

aquaporin; cerebral microvessels; reverse transcription-polymerase chain reaction; water permeability;

D O I：

10.1016/S0304-3940(00)01705-5

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We investigated the expression of aquaporin (AQP) subtypes in the rat cerebral microvessels by reverse transcription-polymerase chain reaction, immunoblotting and immunohistochemistry. mRNA for AQP4, but not for AQP1, 2, 3 or 5, was detected in the microvessels. Immunoblot analysis showed that AQP4 protein was detected as a 30 kDa band with higher molecular weight bands. Immunohistochemical staining showed that AQP4 was located on cell surface of the cerebral microvessels. These results suggest that AQP4 in the cerebral microvessels is involved in the regulation of water transport between blood and brain. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

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页码：163 / 166

页数：4

相关论文

共 21 条

[1] Epithelial aquaporins [J].

Deen, PMT ;

van Os, CH .

CURRENT OPINION IN CELL BIOLOGY, 1998, 10 (04) :435-442

[2] IMMUNOLOCALIZATION OF THE MERCURIAL-INSENSITIVE WATER CHANNEL AND GLYCEROL INTRINSIC PROTEIN IN EPITHELIAL-CELL PLASMA-MEMBRANES [J].

FRIGERI, A ;

GROPPER, MA ;

TURCK, CW ;

VERKMAN, AS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (10) :4328-4331

[3] MOLECULAR-CLONING AND EXPRESSION OF A MEMBER OF THE AQUAPORIN FAMILY WITH PERMEABILITY TO GLYCEROL AND UREA IN ADDITION TO WATER EXPRESSED AT THE BASOLATERAL MEMBRANE OF KIDNEY COLLECTING DUCT CELLS [J].

ISHIBASHI, K ;

SASAKI, S ;

FUSHIMI, K ;

UCHIDA, S ;

KUWAHARA, M ;

SAITO, H ;

FURUKAWA, T ;

NAKAJIMA, K ;

YAMAGUCHI, Y ;

GOJOBORI, T ;

MARUMO, F .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (14) :6269-6273

[4]

KLATZO I, 1994, ACTA NEUROCHIR, P3

[5] ONTOGENESIS OF ALPHA-RECEPTORS AND BETA-RECEPTORS LOCATED ON CEREBRAL MICRO-VESSELS [J].

KOBAYASHI, H ;

CAZZANIGA, A ;

SPANO, P ;

TRABUCCHI, M .

BRAIN RESEARCH, 1982, 242 (02) :358-360

[6] IDENTIFICATION OF BETA-ADRENERGIC-RECEPTOR BINDING-SITES IN RAT-BRAIN MICRO-VESSELS, USING [I-125]IODOHYDROXYBENZYLPINDOLOL [J].

KOBAYASHI, H ;

MEMO, M ;

SPANO, PF ;

TRABUCCHI, M .

JOURNAL OF NEUROCHEMISTRY, 1981, 36 (04) :1383-1388

[7] EFFECT OF AGE ON BETA-ADRENERGIC RECEPTORS ON CEREBRAL MICRO-VESSELS [J].

KOBAYASHI, H ;

MAORET, T ;

SPANO, PF ;

TRABUCCHI, M .

BRAIN RESEARCH, 1982, 244 (02) :374-377

[8]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[9] The human AQP4 gene: Definition of the locus encoding two water channel polypeptides in brain [J].

Lu, MQ ;

Lee, MD ;

Smith, BL ;

Jung, JS ;

Agre, P ;

Verdijk, MAJ ;

Merkx, G ;

Rijs, JPL ;

Deen, PMT .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (20) :10908-10912

[10] HYPERTENSION REDUCES THE NUMBER OF BETA-ADRENERGIC RECEPTORS IN RAT-BRAIN MICROVESSELS [J].

MAGNONI, MS ;

KOBAYASHI, H ;

CAZZANIGA, A ;

IZUMI, F ;

SPANO, PF ;

TRABUCCHI, M .

CIRCULATION, 1983, 67 (03) :610-613