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Genome-wide discovery and characterization of microsatellite markers from Melipona fasciculata (Hymenoptera: Apidae), cross-amplification and a snapshot assessment of the genetic diversity in two stingless bee populations
被引:3
|作者:
Da Silva, Geice Ribeiro
[1
]
De Brito Souza, Isis Gomes
[2
]
Pereira, Fabia De Mello
[3
]
Souza, Bruno De Almeida
[3
]
Do Rego Lopes, Maria Teresa
[3
]
Bentzen, Paul
[4
]
Diniz, Fabio Mendonca
[3
,5
]
机构:
[1] Univ Fed Piaui, Campus Univ Minist Petronio Portella, BR-64049550 Teresina, PI, Brazil
[2] Univ Fed Piaui, Northeast Biotechnol Network RENORBIO, BR-64049550 Teresina, PI, Brazil
[3] EMBRAPA Meio Norte, Lab Mol Biol & Biotechnol, CP 01, BR-64006220 Teresina, PI, Brazil
[4] Dalhousie Univ, Dept Biol, Halifax, NS B3H 4J1, Canada
[5] EMBRAPA Caprinos & Ovinos, Caixa Postal 145, BR-62010970 Sobral, CE, Brazil
关键词:
Hymenoptera;
Apidae;
Meliponini;
Melipona fasciculata;
molecular markers;
microsatellites;
population differentiation;
population structure;
Brazil;
LOCI;
DIFFERENTIATION;
POLLINATORS;
PROGRAM;
CROPS;
SOFTWARE;
BRAZIL;
SMITH;
STATE;
D O I:
10.14411/eje.2018.058
中图分类号:
Q96 [昆虫学];
学科分类号:
摘要:
Brazilian native meliponines are currently threatened by increased human impacts. The assessment of their genetic variation by microsatellite DNA markers can assist in the conservation of populations and help in the planning and establishment of efficient management strategies. The purpose of this study was to develop the first set of microsatellite markers for Melipona fasciculata, selected from partial genome assembly of Illumina paired-end reads. Primer pairs were designed for each detected locus at their flanking regions. Bee samples were genotyped from two different populations of Northeastern Brazil for marker characterization and validation. A total of 17 microsatellite loci displayed polymorphism. Mean H-E and H(O )heterozygosities were 0.453 and 0.536, respectively. PIC across all loci ranged from 0.108 to 0.714. A genetic diversity analysis revealed high values for population differentiation estimates (F-ST = 0.194, R-ST = 0.230, and D-est = 0.162) within the investigated region. PCoA and Bayesian clustering showed a separation of the species into two distinct clusters. These microsatellite markers have demonstrated strong potential for population-level genetic studies. Moreover, the preliminary analysis of the genetic diversity in M. fasciculata provides provisional evidence of significant population differentiation between the two studied populations.
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页码:614 / 619
页数:6
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