Plasmid profile and role in virulence of salmonella enterica serovars isolated from food animals and humans in Lagos Nigeria

被引：4
作者：
Abraham, Ajayi [1 ]
Ifeanyi, Smith Stella [2 ]
Muinah, Fowora [2 ]
Oreoluwa, Bode-Sojobi Ibidunni [3 ]
Coulibaly, Kalpy Julien [4 ]
Adeyemi, Adeleye Isaac [1 ]
机构：
[1] Univ Lagos Akoka, Dept Microbiol, Lagos, Nigeria
[2] Nigerian Inst Med Res Lagos, Mol Biol & Biotechnol Dept, Lagos, Nigeria
[3] Lagos Univ Teaching Hosp, Dept Med Microbiol, Lagos, Nigeria
[4] Inst Pasteur Cote dIvoire, Lab Bacteriol & Virol, Ctr Natl Reference Salmonella, Abidjan, Cote Ivoire
来源：
PATHOGENS AND GLOBAL HEALTH | 2019年 / 113卷 / 06期
关键词：
Plasmid; serovar; salmonella; virulence; ciprofloxacin; conjugation;
D O I：
10.1080/20477724.2019.1691364
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
Infections caused by non-typhoidal Salmonella (NTS) are common around the world, with high morbidity and mortality rates recorded annually. Salmonella serovars harbor plasmids of various sizes which may play roles in antibiotic resistance and virulence. The aim of this study was to profile and determine the role of plasmids in ciprofloxacin resistance and virulence of Salmonella serovars. Using alkaline lysis method 25 NTS serovars from food animals and humans were assayed for plasmids. Isolates ability to resist healthy human serum, bind congo red, produce hemolysin and susceptibility to ciprofloxacin before and after plasmid curing were evaluated. Mobility of plasmids was determined by conjugation. Fifteen (60%) of the 25 Salmonella serovars harbored plasmids with sizes ranging from 0.4 to 38.4 kb. S. Budapest serovars harbored 5-9 plasmids, while S. Essen and S. Mura had six plasmids each. S. Chomedey and a S. Budapest serovar were sensitive to ciprofloxacin after plasmid curing while other serovars remained resistant to ciprofloxacin after plasmid curing. All Salmonella isolates had the ability to withstand human serum before and after plasmid curing, however, some serovars lost their ability to bind congo red after plasmid curing. All Salmonella isolates that initially displayed hemolysin activity retained their ability after curing. Thirteen (86.7%) of the 15 serovars that harbored plasmids conjugatively transferred their plasmids to E. coli K-12 (DH5 alpha). Having Salmonella serovars that harbor transferrable plasmids in the food chain can drive antibiotic resistance and enhanced virulence of otherwise less virulence strains.
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页码：282 / 287
页数：6
相关论文
共 24 条
  • [1] Kuang D., Zhang J., Xu X., Et al., Emerging high-level ciprofloxacin resistance and molecular basis of resistance in Salmonella enterica from humans, food and animals, Int J Food Microbiol, 280, pp. 1-9, (2018)
  • [2] Okoro C.K., Kingsley R.A., Connor T.R., Et al., Intra-continental spread of human invasive Salmonella Typhimurium pathovariants in sub-Saharan Africa, Nat Genet, 44, 11, pp. 1215-1221, (2012)
  • [3] Fernandez J., Guerra B., Rodicio M.R., Resistance to carbapenems in non-typhoidal Salmonella enterica serovars from human, animals and food, Vet Sci, 5, (2018)
  • [4] Kaur J., Jain S.K., Role of antigens and virulence factors of Salmonella enterica serovar Typhi in its pathogenesis, Microbiol Res, 167, pp. 199-210, (2012)
  • [5] Rychlik I., Gregorova D., Hradecka H., Distribution and function of plasmids in Salmonella enterica, Vet Microbiol, 112, pp. 1-10, (2006)
  • [6] Klemm E.J., Shakoor S., Page A.J., Et al., Emergence of an extensively drug-resistant Salmonella enterica serovar Typhi clone harboring a promiscuous plasmid encoding resistance to fluoroquinolones and third generation cephalosporins, mBio, 9, pp. e00105-e00118, (2018)
  • [7] Santos R.L., Tsolis R.M., Baumler A.J., Et al., Pathogenesis of Salmonella induced enteritis, Braz J Med Biol Res, 36, pp. 3-12, (2003)
  • [8] Heffernan E.J., Reed S., Hackett J., Et al., Mechanism of resistance to complement-mediated killing of bacteria encoded by the Salmonella Tyhimurium virulence plasmid gene rck, J Clin Invest, 90, 3, pp. 953-964, (1992)
  • [9] Huang L.J., Cui J., Piao H.H., Et al., Molecular cloning and characterization of clyA genes in various serotypes of Salmonella enterica, J Microbiol, 48, 5, pp. 663-667, (2010)
  • [10] Mezal E.H., Stefanova R., Khan A.A., Isolation and molecular characterization of Salmonella enterica serovar javiana from food, environment and clinical samples, Int J Food Microbiol, 164, pp. 113-118, (2013)
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