A novel extracellular peroxidase and nucleases from a milky sap of Chelidonium majus

被引：21
作者：
Nawrot, Robert
Lesniewicz, Krzysztof
Pienkowska, Joanna
Gozdzicka-Jozefiak, Anna
机构：
[1] Adam Mickiewicz Univ Poznan, Inst Expt Biol, Dept Mol Virol, PL-61614 Poznan, Poland
[2] Adam Mickiewicz Univ Poznan, Inst Mol Biol & Biotechnol, Dept Mol & Cellular Biol, PL-60317 Poznan, Poland
[3] Adam Mickiewicz Univ Poznan, Inst Expt Biol, Dept Cell Biol, PL-61614 Poznan, Poland
来源：
FITOTERAPIA | 2007年 / 78卷 / 7-8期
关键词：
Chelidonium majus; peroxidase; nuclease;
D O I：
10.1016/j.fitote.2007.04.012
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Using affinity chromatography, SDS-PAGE, peroxidase activity assay and mass spectrometry data, a new extracellular peroxidase (CMP) from Chelidonium majus milky sap was isolated and characterized. The protein has a molecular weight of about 40 kDa and belongs to secretory class III plant peroxidases. The peroxidase activity is also accompanied by DN-ase activities. A novel CNIP combined with other proteins is probably involved in development and differentiation of the plant and defence against different pathogens. It suggests that the biological activity of C. majus whole plants and extracts may depend not only on its alkaloidal content but also on the presence of biologically active proteins. (c) 2007 Elsevier 13N. All rights reserved.
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收藏
页码：496 / 501
页数：6
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