Enhancing protein to extremely high content in photosynthetic bacteria during biogas slurry treatment

被引：43

作者：

Yang, Anqi ^{[1
]}

Zhang, Guangming ^{[1
]}

Meng, Fan ^{[1
]}

Lu, Pei ^{[2
]}

Wang, Xintian ^{[1
]}

Peng, Meng ^{[1
]}

机构：

[1] Renmin Univ China, Sch Environm & Nat Resource, 59 Zhongguancun St, Beijing 100872, Peoples R China

[2] Univ Sci & Technol Beijing, Sch Energy & Environm Engn, 30 Xueyuan Rd, Beijing 100083, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2017年 / 245卷

关键词：

Protein; Photosynthetic bacteria; Biogas slurry; Enhancement; SINGLE-CELL PROTEIN; SALINITY STRESS; WASTE-WATER; PATHWAYS; NITROGEN;

D O I：

10.1016/j.biortech.2017.08.109

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

This work proposed a novel approach to achieve an extremely high protein content in photosynthetic bacteria (PSB) using biogas slurry as a culturing medium. The results showed the protein content of PSB could be enhanced strongly to 90% in the biogas slurry, which was much higher than reported microbial protein contents. The slurry was partially purified at the same time. Dark-aerobic was more beneficial than light-anaerobic condition for protein accumulation. High salinity and high ammonia of the biogas slurry were the main causes for protein enhancement. In addition, the biogas slurry provided a good buffer system for PSB to grow. The biosynthesis mechanism of protein in PSB was explored according to theoretical analysis. During biogas slurry treatment, the activities of glutamate synthase and glutamine synthetase were increased by 26.55%, 46.95% respectively.

引用

页码：1277 / 1281

页数：5

共 13 条

[1] DETERMINATION OF AMMONIA BY NESSLER METHOD IN WATERS CONTAINING HYDRAZINE [J].

CROSBY, NT .

ANALYST, 1968, 93 (1107) :406-&

[2] Pathways of nitrogen utilization by soil microorganisms - A review [J].

Geisseler, Daniel ;

Horwath, William R. ;

Joergensen, Rainer Georg ;

Ludwig, Bernard .

SOIL BIOLOGY & BIOCHEMISTRY, 2010, 42 (12) :2058-2067

[3]

Greensberg A.E., 1992, American Public Health Association/American Water Works Association/Water Environment Federation, V18, P108, DOI DOI 10.1016/J.RESCONREC.2017.11.002

[4]

Jo JH, 2012, CAN J MICROBIOL, V58, P278, DOI [10.1139/w11-132, 10.1139/W11-132]

[5]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[6] Influences of light and oxygen conditions on photosynthetic bacteria macromolecule degradation: Different metabolic pathways [J].