Comparison of a range of green fluorescent protein-tagging vectors for monitoring a microbial inoculant in soil

被引：21

作者：

Dandie, CE ^{[1
]}

Thomas, SM ^{[1
]}

McClure, NC ^{[1
]}

机构：

[1] Flinders Univ S Australia, Sch Biol Sci, Adelaide, SA 5001, Australia

来源：

LETTERS IN APPLIED MICROBIOLOGY | 2001年 / 32卷 / 01期

关键词：

D O I：

10.1046/j.1472-765x.2001.00848.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two new transposon-based tagging vectors have been constructed using the gfp marker gene under control of either constitutive or inducible promoters. The two vectors, along with the established pUTminiTn5gfp were used to tag a diesel-degrading Pseudomonas strain. Tagged strains were obtained that were not affected in terms of their growth or ability to use diesel as a carbon source. The transposon tags were stably maintained in the strains without selection and provided visible fluorescence as colonies or single cells in suspension. Tagging did not impede the survival of tagged Pseudomonas aeruginosa GP41B strains in diesel-contaminated soil microcosms. The tagged strains were easily recovered from the microcosms after a 3-month period. The tagging of bacteria with gfp using either native or introduced constitutive/inducible promoters is an effective and easy way to monitor their survival in soil.

引用

页码：26 / 30

页数：5

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