The probability of achieving full allelic representation for LCN-STR profiling of haploid cells

被引：18

作者：

Lucy, D. ^{[1
]}

Curtan, J. M. ^{[2
]}

Pirie, A. A. ^{[3
]}

Gill, P. ^{[4
]}

机构：

[1] Univ Lancaster, Dept Math & Stat, Lancaster LA1 4YF, England

[2] Univ Auckland, Dept Stat, Auckland 1010, New Zealand

[3] Lothian & Borders Police Forens Sci Lab, Edinburgh EH16 6TF, Midlothian, Scotland

[4] Forens Sci Serv Inc, Birmingham B37 7YN, W Midlands, England

来源：

SCIENCE & JUSTICE | 2007年 / 47卷 / 04期

基金：

英国经济与社会研究理事会;

关键词：

haploid cells; sample size;

D O I：

10.1016/j.scijus.2007.10.001

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

Modem forensic techniques allow DNA to be extracted from ever decreasing amounts of cellular material. Low copy number (LCN) profiling enables the production of STR profiles from small numbers of cells. Moreover, methods such as laser micro-dissection enables forensic scientists to potentially isolate individual cells for PCR. The DNA derived from haploid cells (semen) is a common source of forensic evidence in sexual assault cases. Haploid cells contain only half the DNA complement of diploid cells (3 pg compared to 6 pg). The smaller the number of cells sampled, the smaller the probability that there is a full representation of the alleles comprising the donor profile. This paper investigates the relationship between the number of cells sampled and the probability of full representation of all alleles in the donor sample. It also considers the effect of typing several loci as opposed to just a single locus. (c) 2007 Forensic Science Society. Published by Elsevier Ireland Ltd. All rights reserved.

引用

页码：168 / 171

页数：4

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