Tutorial review on validation of liquid chromatography-mass spectrometry methods: Part II

被引：198

作者：

Kruve, Anneli ^{[1
]}

Rebane, Riin ^{[1
]}

Kipper, Karin ^{[1
]}

Oldekop, Maarja-Liisa ^{[1
]}

Evard, Hanno ^{[1
]}

Herodes, Koit ^{[1
]}

Ravio, Pekka ^{[2
]}

Leito, Ivo ^{[1
]}

机构：

[1] Univ Tartu, Inst Chem, EE-50411 Tartu, Estonia

[2] Finnish Customs Lab, FI-02151 Espoo, Finland

来源：

ANALYTICA CHIMICA ACTA | 2015年 / 870卷

关键词：

Liquid chromatography-mass spectrometry; Validation; Electrospray; Ionization suppression; Limit of detection; Robustness; LC-MS/MS METHOD; MULTI-RESIDUE ANALYSIS; BIOANALYTICAL METHOD VALIDATION; SINGLE-LABORATORY VALIDATION; ELECTROSPRAY-IONIZATION; HUMAN PLASMA; PESTICIDE-RESIDUES; HUMAN URINE; PHARMACEUTICAL ANALYSIS; MEASUREMENT UNCERTAINTY;

D O I：

10.1016/j.aca.2015.02.016

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This is the part II of a tutorial review intending to give an overview of the state of the art of method validation in liquid chromatography mass spectrometry (LC-MS) and discuss specific issues that arise with MS (and MS-MS) detection in LC (as opposed to the "conventional" detectors). The Part II starts with briefly introducing the main quantitation methods and then addresses the performance related to quantification: linearity of signal, sensitivity, precision, trueness, accuracy, stability and measurement uncertainty. The last section is devoted to practical considerations in validation. With every performance characteristic its essence and terminology are addressed, the current status of treating it is reviewed and recommendations are given, how to handle it, specifically in the case of LC-MS methods. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：8 / 28

页数：21

共 144 条

[1] Linear regression for calibration lines revisited: weighting schemes for bioanalytical methods [J].