Sargassum fusiforme fucoidan ameliorates diet-induced obesity through enhancing thermogenesis of adipose tissues and modulating gut microbiota

Obesity has become a global epidemic. Sargassum fusiforme fucoidan (Fuc) is a group of water-soluble hetero-polysaccharides that exhibits a wide range of medicinal functions. It consists of L-fucose and sulfate groups, with L-fucose as the main monosaccharide. This study investigated the therapeutic effects of Fuc on diet-induced obesity (DIO) in C57BL/6J female mice. Fuc significantly alleviated obesity in mice induced by high-fat high -fructose (HFHF) feeding, inhibiting body weight gain, reducing fat accumulation, and improving hepatic stea-tosis. In addition, Fuc significantly improved glucose tolerance and insulin sensitivity by enhancing the phos-phorylation level of AKT (at Ser473) in the adipose tissues. Mechanistically, although Fuc did not decrease the energy intake in DIO mice, it significantly increased the energy expenditure by up-regulating the expression of uncoupling protein 1 (UCP1) in the adipose tissues. Notably, Fuc also improved the obesity-driven dysbiosis of gut microbiota and decreased the relative abundance of the obesity-related intestinal bacteria. However, Fuc was unable to alleviate DIO-induced metabolic disorders in pseudo-sterile mice. Our findings suggested that Fuc might remodel gut microbiota and exert its weight loss and hypolipidemic effects by increasing the energy expenditure, thus providing a novel perspective for treating obesity and related complications.