Molecular characterization and analysis of conserved potyviral motifs in bean common mosaic virus (BCMV) for RNAi-mediated protection

被引：29
作者：
Worrall, Elizabeth A. [1 ]
Hayward, Alice C. [1 ]
Fletcher, Stephen J. [1 ]
Mitter, Neena [1 ]
机构：
[1] Univ Queensland, Ctr Hort Sci, Queensland Alliance Agr & Food Innovat, Brisbane, Qld 4072, Australia
来源：
ARCHIVES OF VIROLOGY | 2019年 / 164卷 / 01期
关键词：
COMPLETE GENOME SEQUENCE; COMPLETE NUCLEOTIDE-SEQUENCE; NECROSIS-VIRUS; COAT PROTEIN; PHASEOLUS-VULGARIS; 1ST REPORT; RESISTANCE; IDENTIFICATION; TRANSMISSIBILITY; RECOMBINATION;
D O I：
10.1007/s00705-018-4065-6
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Australian bean common mosaic virus (BCMV) isolates were sequenced, and the sequences were compared to global BCMV and bean common mosaic necrosis virus (BCMNV) sequences and analysed for conserved potyviral motifs to generate in planta RNA-interference (RNAi) resistance. Thirty-nine out of 40 previously reported potyvirus motifs were conserved among all 77 BCMV/BCMNV sequences. Two RNAi target regions were selected for dsRNA construct design, covering 920 bp of the nuclease inclusion b (NIb) protein and 461 bp of the coat protein (CP). In silico prediction of the effectiveness of these constructs for broad-spectrum defence against the 77 BCMV and BCMNV sequences was done via analysis of putative 21-nucleotide (nt) and 22-nt small-interfering RNAs (siRNAs) generated from the target regions. The effectiveness of both constructs for siRNA generation and BCMV RNAi-mediated resistance was validated in Nicotiana benthamiana transient assays.
引用
收藏
页码：181 / 194
页数：14
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