Image fusion of IR and optical microscopy for mapping of biomolecules in tissue

被引：5

作者：

Al Jedani, Safaa ^{[1
]}

Whitley, Conor A. ^{[1
]}

Ellis, Barnaby G. ^{[1
]}

Triantafyllou, Asterios ^{[2
]}

Smith, Caroline I. ^{[1
]}

Gunning, Philip J. ^{[3
]}

Gardner, Peter ^{[4
,5
]}

Risk, Janet M. ^{[3
]}

Weightman, Peter ^{[1
]}

Barrett, Steve D. ^{[1
]}

机构：

[1] Univ Liverpool, Dept Phys, Liverpool L69 7ZE, Merseyside, England

[2] Univ Liverpool, Liverpool Clin Labs, Dept Pathol, Liverpool L69 3GA, Merseyside, England

[3] Univ Liverpool, Inst Syst Mol & Integrat Biol, Dept Mol & Clin Canc Med, Liverpool L3 9TA, Merseyside, England

[4] Manchester Inst Biotechnol, 131 Princess St, Manchester M1 7DN, Lancs, England

[5] Univ Manchester, Manchester M1 7DN, Lancs, England

来源：

ANALYST | 2021年 / 146卷 / 19期

基金：

英国工程与自然科学研究理事会;

关键词：

MULTISCALE; MS;

D O I：

10.1039/d1an01161h

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

It is shown that a pixel-level image fusion technique can produce images that combine the spatial resolution of optical microscopy images of haematoxylin and eosin (H&E) stained tissue with the chemical information in Fourier transform infrared (FTIR) images. The fused images show minimal distortion and the higher spatial resolution of the H&E images overcomes the diffraction limit on the spatial resolution of the FTIR images. A consideration of the FTIR spectra of nucleic acids and collagen can explain the changes in contrast between non-cancerous oral epithelium and underlying stroma within fused images formed by combining an H&E stain of oral tissue with FTIR images of the tissue obtained at a number of wavenumbers.

引用

页码：5848 / 5854

页数：7

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