Effects of vaccine-acquired polyclonal anti-HBs antibodies on the prevention of HBV infection of non-vaccine genotypes

被引：17

作者：

Kato, Masaki ^{[1
,2
]}

Hamada-Tsutsumi, Susumu ^{[3
,4
]}

Okuse, Chiaki ^{[5
]}

Sakai, Aiko ^{[6
]}

Matsumoto, Nobuyuki ^{[2
]}

Sato, Masaaki ^{[7
]}

Sato, Toshiyuki ^{[7
]}

Arito, Mitsumi ^{[7
]}

Omoteyama, Kazuki ^{[7
]}

Suematsu, Naoya ^{[7
]}

Okamoto, Kazuki ^{[7
]}

Kato, Takanobu ^{[8
]}

Itoh, Fumio ^{[2
]}

Sumazaki, Ryo ^{[6
]}

Tanaka, Yasuhito ^{[3
,4
]}

Yotsuyanagi, Hiroshi ^{[9
]}

Kato, Tomohiro ^{[7
]}

Kurokawa, Manae Suzuki ^{[1
]}

机构：

[1] St Marianna Univ, Dis Biomarker Anal & Mol Regulat, Grad Sch Med, Miyamae Ku, 2-16-1 Sugao, Kawasaki, Kanagawa 2168511, Japan

[2] St Marianna Univ, Div Gastroenterol & Hepatol, Dept Internal Med, Sch Med, Kawasaki, Kanagawa, Japan

[3] Nagoya City Univ, Dept Virol, Grad Sch Med Sci, Nagoya, Aichi, Japan

[4] Nagoya City Univ, Liver Unit, Grad Sch Med Sci, Nagoya, Aichi, Japan

[5] Kawasaki Municipal Tama Hosp, Div Gastroenterol & Hepatol, Kawasaki, Kanagawa, Japan

[6] Univ Tsukuba, Dept Child Hlth, Fac Med, Tsukuba, Ibaraki, Japan

[7] St Marianna Univ, Clin Prote & Mol Med, Grad Sch Med, Kawasaki, Kanagawa, Japan

[8] Natl Inst Infect Dis, Dept Virol 2, Tokyo, Japan

[9] Univ Tokyo, Grad Sch Med, Dept Infect Dis, Tokyo, Japan

来源：

JOURNAL OF GASTROENTEROLOGY | 2017年 / 52卷 / 09期

关键词：

Hepatitis B virus; Genotypes; Universal vaccination; Polyclonal anti-HBs antibodies; HEPATITIS-B-VIRUS; SURFACE-ANTIGEN; TRANSMISSION; CHILDREN; JAPAN; PERSISTENCE;

D O I：

10.1007/s00535-017-1316-3

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Background In universal hepatitis B (HB) vaccination, single vaccine-derived polyclonal anti-HBs antibodies (anti-HBs) need to inhibit infection of HB viruses (HBV) of non-vaccine genotypes. We experimentally addressed this issue. Methods Anti-HBs-positive sera were obtained by vaccination with genotype A- or C-derived HBs antigen (HBsAg, gtA-sera or gtC-sera). Their reactivity to genotype A- and C-derived HBsAg (gtA-Ag and gtC-Ag) was measured by ELISA. The capacity of sera to neutralize HBV was evaluated using an in vitro infection model. Results Of 135 anti-gtA-Ag-reactive gtA-sera, 134 (99.3%) were anti-gtC-Ag-reactive. All (100%) 120 anti-gtC-Ag-reactive gtC-sera were anti-gtA-Ag-reactive. The reactivity to gtA-Ag was strongly correlated with that to gtC-Ag (gtA-sera, rho = 0.989; gtC-sera, rho = 0.953; p < 0.01). In gtA-sera (n = 10), anti-HBs to gtA-Ag were less completely absorbed with gtC-Ag (96.4%) than with gtA-Ag (100%, p < 0.05). Similarly, in gtC-sera (n = 10), anti-HBs to gtC-Ag were less completely absorbed with gtA-Ag (96.0%) than with gtC-Ag (100%, p < 0.01). Thus, 3.6 and 4.0% of anti-HBs in gtA-sera and gtC-sera were vaccine genotype HBsAg-specific, respectively. In the neutralization test, gtA-sera (n = 4) and gtC-sera (n = 3) with anti-HBs titers adjusted to 100 mIU/mL equally inhibited genotype C HBV infection (92.8 vs. 95.4%, p = 0.44). However, at 30 mIU/mL, the gtA-sera less effectively inhibited infection than the gtC-sera (60.2 vs. 90.2%, p < 0.05). Conclusion Vaccination with genotype A- or C-derived HBsAg provided polyclonal anti-HBs that sufficiently bound to non-vaccine genotype HBsAg. However, a small portion of anti-HBs were specific to the vaccine genotype HBsAg. High anti-HBs titers would be required to prevent HBV infection of non-vaccine genotypes. UMIN/CTR UMIN000014363.

引用

页码：1051 / 1063

页数：13

共 36 条

[1]

[Anonymous], 2016, HEP B

[2] Amino acid substitutions in the S region of hepatitis B virus in sera from patients with acute hepatitis [J].

Aono, Junko ;

Yotsuyanagi, Hiroshi ;

Miyoshi, Hideyuki ;

Tsutsumi, Takeya ;

Fujie, Hajime ;

Shintani, Yoshizumi ;

Moriya, Kyoji ;

Okuse, Chiaki ;

Suzuki, Michihiro ;

Yasuda, Kiyomi ;

Iino, Shiro ;

Koike, Kazuhiko .

HEPATOLOGY RESEARCH, 2007, 37 (09) :731-739

[3] Hepatitis B virus transmission pattern and vaccination efficiency in Uzbekistan [J].

Avazova, Dildora ;

Kurbanov, Fuat ;

Tanaka, Yasuhito ;

Sugiyama, Masaya ;

Radchenko, Ivan ;

Ruziev, Dilmurod ;

Musabaev, Erkin ;

Mizokami, Masashi .

JOURNAL OF MEDICAL VIROLOGY, 2008, 80 (02) :217-224

[4]

Banatvala J, 2000, LANCET, V355, P561, DOI 10.1016/S0140-6736(99)07239-6

[5]

Cassidy A, 2011, EXPERT REV VACCINES, V10, P1709, DOI [10.1586/ERV.11.151, 10.1586/erv.11.151]

[6] Persistence of long-term immunity to hepatitis B among adolescents immunized at birth [J].

Chaves, Sandra S. ;

Fischer, Gayle ;

Groeger, Justina ;

Patel, Priti R. ;

Thompson, Nicola D. ;

Teshale, Eyasu H. ;

Stevenson, Kuartei ;

Yano, Victor M. ;

Armstrong, Gregory L. ;

Samandari, Taraz ;

Kamili, Saleem ;

Drobeniuc, Jan ;

Hu, Dale J. .

VACCINE, 2012, 30 (09) :1644-1649

[7] Seroepidemiology of hepatitis B virus infection in children - Ten years of mass vaccination in Taiwan [J].

Chen, HL ;

Chang, MH ;

Ni, YH ;

Hsu, HY ;

Lee, PI ;

Lee, CY ;

Chen, DS .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1996, 276 (11) :906-908

[8] Hepatitis B seroprevalence in Thailand: 12 years after hepatitis B vaccine integration into the national expanded programme on immunization [J].

Chongsrisawat, Voranush ;

Yoocharoen, Pornsak ;

Theamboonlers, Apiradee ;

Tharmaphornpilas, Piyanit ;

Warinsathien, Porpit ;

Sinlaparatsamee, Supakarn ;

Paupunwatana, Siriraj ;

Chaiear, Kasemporn ;

Khwanjaipanich, Sawan ;

Poovorawan, Yong .

TROPICAL MEDICINE & INTERNATIONAL HEALTH, 2006, 11 (10) :1496-1502

[9] Selective Hepatitis B Virus Vaccination Has Reduced Hepatitis B Virus Transmission in The Netherlands [J].

Hahne, Susan ;

van Houdt, Robin ;

Koedijk, Femke ;

van Ballegooijen, Marijn ;

Cremer, Jeroen ;

Bruisten, Sylvia ;

Coutinho, Roel ;

Boot, Hein .

PLOS ONE, 2013, 8 (07)

[10] Validation of Cross-Genotype Neutralization by Hepatitis B Virus-Specific Monoclonal Antibodies by In Vitro and In Vivo Infection [J].

Hamada-Tsutsumi, Susumu ;

Iio, Etsuko ;

Watanabe, Tsunamasa ;

Murakami, Shuko ;

Isogawa, Masanori ;

Iijima, Sayuki ;

Inoue, Takako ;

Matsunami, Kayoko ;

Tajiri, Kazuto ;

Ozawa, Tatsuhiko ;

Kishi, Hiroyuki ;

Muraguchi, Atsushi ;

Joh, Takashi ;

Tanaka, Yasuhito .

PLOS ONE, 2015, 10 (02)

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