Determination of macrolides in biological matrices by high-performance liquid chromatography with electrochemical detection

被引：117

作者：

Kees, F ^{[1
]}

Spangler, S ^{[1
]}

Wellenhofer, M ^{[1
]}

机构：

[1] Univ Regensburg, Inst Pharm, D-93053 Regensburg, Germany

来源：

JOURNAL OF CHROMATOGRAPHY A | 1998年 / 812卷 / 1-2期

关键词：

macrolides; antibiotics;

D O I：

10.1016/S0021-9673(97)01279-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A liquid chromatographic method for the determination of macrolide antibiotics is described using a cyanopropyl column which proved to be as efficient or superior to the normally used apolar reversed-phase columns. The recovery of the macrolides from water and plasma was 80-90%. Using 0.5 mi of plasma, 30 ng/ml of clarithromycin, 50 ng/ml of roxithromycin and 10 ng/ml of azithromycin could be determined with acceptable precision and accuracy. The method has been employed in pharmacokinetic studies in humans for the determination of roxithromycin, clarithromycin and azithromycin in plasma, serum and other biological matrices. The particular selectivity of the cyanopropyl phase may also allow the simultaneous determination of erythromycin and its prodrug esters. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：287 / 293

页数：7

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