"Solvent-free" ultrasound-assisted extraction of lipids from fresh microalgae cells: A green, clean and scalable process

被引：198

作者：

Adam, Fanny ^{[1
]}

Abert-Vian, Maryline ^{[1
]}

Peltier, Gilles ^{[2
]}

Chemat, Farid ^{[1
]}

机构：

[1] Univ Avignon & Pays de Vaucluse, INRA, UMR408, F-84000 Avignon, France

[2] Univ Aix Marseille, CEA,CEA Cadarache, CNRS,Inst Biol Environm & Biotechnol,UMR 6191, Lab Bianerget & Biotechnol Bacteries & Microalgue, F-13108 St Paul Les Durance, France

来源：

BIORESOURCE TECHNOLOGY | 2012年 / 114卷

关键词：

Ultrasound-assisted extraction (UAE); Bio-oil; Response surface methodology (RSM); Nannochloropsis sp; Scanning electron microscopy (SEM)-gas chromatography (GC);

D O I：

10.1016/j.biortech.2012.02.096

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

In order to comply with criteria of green chemistry concepts and sustainability, a new procedure has been performed for solvent-free ultrasound-assisted extraction (UAE) to extract lipids from fresh Nannochloropsis oculata biomass. Through response surface methodology (RSM) parameters affecting the oil recovery were optimized. Optimum conditions for oil extraction were estimated as follows: (i) 1000W ultrasonic power, (ii) 30 min extraction time and (iii) biomass dry weight content at 5%. Yields were calculated by the total fatty acids methyl esters amounts analyzed by GC-FID-MS. The maximum oil recovery was around 0.21%. This value was compared with the one obtained with the conventional extraction method (Bligh and Dyer). Furthermore, effect of temperature on the yield was also investigated. The overall results show an innovative and effective extraction method adapted for microalgae oil recovery, without using solvent and with an enable scaling up. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：457 / 465

页数：9

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