Proteomic analysis of vascular smooth muscle cells in physiological condition and in pulmonary arterial hypertension: Toward contractile versus synthetic phenotypes

被引：27

作者：

Regent, Alexis ^{[1
,2
]}

Ly, Kim Heang ^{[3
]}

Lofek, Sebastien ^{[1
]}

Clary, Guilhem ^{[1
,4
]}

Tamby, Mathieu ^{[1
]}

Tamas, Nicolas ^{[1
]}

Federici, Christian ^{[1
,4
]}

Broussard, Cedric ^{[1
,4
]}

Chafey, Philippe ^{[1
,4
]}

Liaudet-Coopman, Emmanuelle ^{[5
,6
]}

Humbert, Marc ^{[7
,8
,9
]}

Perros, Frederic ^{[7
,8
,9
]}

Mouthon, Luc ^{[1
,2
]}

机构：

[1] Univ Paris 05, LabEx INFLAMEX, CNRS UMR 8104, Inst Cochin,INSERM U1016, Paris, France

[2] Hop Cochin, AP HP, Ctr Reference Vasc Necrosantes & Sclerodermie Sys, Serv Med Interne, Paris, France

[3] CHU Dupuytren, Serv Med Interne A, Limoges, France

[4] Univ Paris 05, Sorbonne Paris Cite, Plate Forme Prote 3P5, Paris, France

[5] IRCM, Montpellier, France

[6] Univ Montpellier, INSERM, U1194, Inst Reg Canc Montpellier, Montpellier, France

[7] Univ Paris Sud, Fac Med, Le Kremlin Bicetre, France

[8] INSERM, UMR S 999, Hypertens Arterielle Pulm Physiopathol & Innovat, LabEx LERMIT, Le Plessis Robinson, France

[9] Ctr Chirurg Marie Lannelongue, Dept Rech Med, Le Plessis Robinson, France

来源：

PROTEOMICS | 2016年 / 16卷 / 20期

关键词：

Cell biology; ELAVL1; Paxillin; Pulmonary hypertension; Vascular smooth muscle cell; ENDOTHELIAL-CELLS; EXPRESSION; ACTIVATION; CANCER; ATHEROSCLEROSIS; PROLIFERATION; MODULATION; DIVERSITY; RESPONSES; BINDING;

D O I：

10.1002/pmic.201500006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Vascular smooth muscle cells (VSMCs) are highly specialized cells that regulate vascular tone and participate in vessel remodeling in physiological and pathological conditions. It is unclear why certain vascular pathologies involve one type of vessel and spare others. Our objective was to compare the proteomes of normal human VSMC from aorta (human aortic smooth muscle cells, HAoSMC), umbilical artery (human umbilical artery smooth muscle cells, HUASMC), pulmonary artery (HPASMC), or pulmonary artery VSMC from patients with pulmonary arterial hypertension (PAH-SMC). Proteomes of VSMC were compared by 2D DIGE and MS. Only 19 proteins were differentially expressed between HAoSMC and HPASMC while 132 and 124 were differentially expressed between HUASMC and HAoSMC or HPASMC, respectively (fold change 1.5 <= or -1.5 >=, p< 0.05). As much as 336 proteins were differentially expressed between HPASMC and PAH-SMC(fold change 1.5 <= or -1.5 >=, p< 0.05). HUASMC expressed increased amount of alpha-smooth muscle actin compared to either HPASMC or HAoSMC (although not statistically significant). In addition, PAH-SMC expressed decreased amount of smooth muscle myosin heavy chain and proliferation rate was increased compared to HPASMC thus supporting that PAH-SMC have a more synthetic phenotype. Analysis with Ingenuity identified paxillin and (embryonic lethal, abnormal vision, drosophila) like 1 (ELAVL1) as molecules linked with a

引用

页码：2637 / 2649

页数：13

共 34 条

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