Nanomolar fluorescent detection of c-di-GMP using a modular aptamer strategy

被引：64

作者：

Nakayama, Shizuka ^{[1
]}

Luo, Yiling ^{[1
]}

Zhou, Jie ^{[1
]}

Dayie, T. Kwaku ^{[1
]}

Sintim, Herman O. ^{[1
]}

机构：

[1] Univ Maryland, Dept Chem & Biochem, College Pk, MD 20742 USA

来源：

CHEMICAL COMMUNICATIONS | 2012年 / 48卷 / 72期

基金：

美国国家科学基金会;

关键词：

CYCLIC DIGUANYLATE; LIGAND-BINDING; RNA; SENSORS; RIBOSWITCH; RECOGNITION; PROTEIN; DYES;

D O I：

10.1039/c2cc34379g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

C-di-GMP regulates important processes involved in biofilm formation and virulence factors production in several bacteria. Herein we report a simple fluorescent strategy that allows for the detection of c-di-GMP (as low as 320 nM) using a Vc2 class I riboswitch domain as the sensing region and spinach as the fluorescent reporting module.

引用

页码：9059 / 9061

页数：3

共 32 条

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