Polydatin combats methotrexate-induced pulmonary fibrosis in rats: Involvement of biochemical and histopathological assessment

被引：9
作者：
Ali, Yomna A. [1 ]
Ahmed, Amany A. E. [2 ]
Abd El-Raouf, Ola M. [1 ]
Elkhoely, Abeer [2 ]
Gad, Amany M. [1 ,3 ]
机构：
[1] Natl Org Drug Control & Res, Egyptian Drug Author EDA, Dept Pharmacol, Cairo, Egypt
[2] Helwan Univ, Fac Pharm, Dept Pharmacol & Toxicol, Cairo, Egypt
[3] Sinai Univ, Fac Pharm, Dept Pharmacol & Toxicol, East Kantara Branch, New City, Egypt
来源：
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY | 2022年 / 36卷 / 05期
关键词：
dexamethasone; methotrexate; oxidative stress; polydatin; pulmonary fibrosis; INDUCED OXIDATIVE STRESS; LUNG INJURY; IN-VIVO; ACTIVATION; COMPLICATIONS; INFLAMMATION; FIBROBLASTS; MECHANISMS; APOPTOSIS;
D O I：
10.1002/jbt.23019
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Polydatin (PD) is a polyphenolic compound found naturally in many fruits such as grapes. It has anti-oxidant and anti-inflammatory activities that are of paramount importance for its pharmacological actions. This study aimed to explore possible protective effects of PD against methotrexate (MTX)-induced pulmonary fibrosis in rats. A single oral dose of MTX (14 mg/kg) per week for 2 weeks caused a significant decrease in glutathione (GSH) content with a marked increase in transforming growth factor-beta (TGF-beta), alpha-smooth muscle actin (alpha-SMA), pulmonary content of malondialdehyde (MDA), interleukin-1 beta (IL-1 beta), Hydroxyproline, tumor necrosis factor-alpha (TNF-alpha), and 8-hydroxy-2'-deoxyguanosine (8-OHdG) as compared with the control group. Contrarily, daily administration of PD (25, 50, and 100 mg/kg, p.o.) for 14 days concomitantly with MTX ameliorated MTX-induced pulmonary fibrosis as indicated by mitigation of the previously mentioned biochemical parameters and histopathological changes in a dose-dependent manner. In conclusion, the protective effect of PD against pulmonary fibrosis induced by MTX in rats might be attributed to its anti-oxidant, anti-inflammatory as well as anti-fibrotic effects.
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页数：9
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