Study on adhesion formation and the healing of colon anastomosis in rats with induced peritoneal sepsis

被引:7
|
作者
Marques e Silva, Silvana
Melo de Oliveira, Marcos Vinicius
Brandao, Alexandre Malta
Carneiro, Fabiana Pirani
Moraes Ferreira, Vania Maria
Parra, Rogerio Serafim
Feres, Omar
de Sousa, Joao Batista
机构
[1] Brasilias Univ Med Sch UNB, Expt Surg Lab, Brasilia, DF, Brazil
[2] Brasilias Univ Med Sch UNB, Pathol Lab, Brasilia, DF, Brazil
[3] Sao Paulos Univ Ribeirao Preto Med Sch USP, Liver Transplant Lab, Sao Paulo, Brazil
关键词
Anastomosis; Surgical; Colon; Sepsis; Tissue Adhesions; Rats; BACTERIAL PERITONITIS; CECAL LIGATION; PREVENTION; MODEL; PUNCTURE;
D O I
10.1590/S0102-86502011000800018
中图分类号
R61 [外科手术学];
学科分类号
摘要
PURPOSE: To evaluate the effects of abdominal sepsis on adhesion formation and colon anastomosis healing in rats. METHODS: Forty rats were distributed in two groups containing 20 rats each for left colon anastomosis in the presence (Group S) or absence (Group N) of induced sepsis by cecal ligation and puncture. Each group was divided into subgroups for euthanasia on the third (N3 and S3) or seventh (N7 or S7) post-operative day. The amount of adhesions was evaluated and a segment of the colon was removed for histopathologic analysis, bursting strength assessment, hydroxyproline and the determination of tissue collagen. RESULTS: The subjects which underwent cecal ligation and puncture presented a higher amount of intra-abdominal adherences in both third (p=0,00) and seventh (p=0,00) post-operatory days. Smaller bursting strengths were found in the S3 subgroup, and greater bursting strengths were found in the S7 subgroup. There was no difference in the variations on the concentrations of hydroxyproline, tissue collagen and histopathology. CONCLUSIONS: The peritoneal infection which was developed by cecal ligation and puncture raised the amount of intra-cavitary adhesions. There was a decrease in the amount of colonic anastomosis on the third post-operatory day with a following raise on the seventh without any effects on other healing parameters.
引用
收藏
页码:100 / 105
页数:6
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