Construction of a high-resolution linkage map of a rice brown planthopper (Nilaparvata lugens Stal) resistance gene bph2

被引：51

作者：

Murai, H

Hashimoto, Z

Sharma, PN

Shimizu, T

Murata, K

Takumi, S

Mori, N

Kawasaki, S

Nakamura, C

机构：

[1] Kobe Univ, Grad Sch Sci & Technol, Lab Plant Genet, Dept Biol & Environm Sci,Fac Agr,Nada Ku, Kobe, Hyogo 6578501, Japan

[2] Kobe Univ, Grad Sch Sci & Technol, Div Life Sci, Nada Ku, Kobe, Hyogo 6578501, Japan

[3] Natl Inst Agrobiol Resources, Tsukuba, Ibaraki 3058602, Japan

来源：

THEORETICAL AND APPLIED GENETICS | 2001年 / 103卷 / 04期

关键词：

brown planthopper (BPH); BPH resistance gene; bph2; AFLP and STS markers; linkage map; rice (Oryza sativa L.);

D O I：

10.1007/s001220100598

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The brown planthopper (BPH), Nilaparvata lugens Stal, is a significant insect pest of rice (Oryza sativa L.). bph2 is one of the 12 major BPH resistance aeries so far identified in several indica cultivars and two wild relatives. We have constructed a high-resolution linkage map as a foundation for map-based cloning of the bph2 locus. An advanced mapping population derived from a cross of 'Tsukushibare' (a susceptible japonica cultivar) with 'Norin-PL4' (an authentic bph2-introgression line) was used. Segregation analysis by the mass seedling test showed that bph2 behaved as a single dominant gene. Through bulked segregant analysis and linkage analysis, bph2 was located within a 3.2-cM region containing eight AFLP markers. One marker (KAM4) showed complete co-segregation with bph2, and bph2 was mapped within a 1.0-cM region delimited by KAM3 and KAM5, two flanking markers. KAM4 was converted into a PCR-based sequence-tagged-site (STS) marker and its co-segregation with bph2 was validated.

引用

页码：526 / 532

页数：7