The CD133+ tumor stem-like cell-associated antigen may elicit highly intense immune responses against human malignant glioma

被引：31

作者：

Hua, Wei ^{[1
]}

Yao, Yu ^{[1
]}

Chu, Yiwei ^{[2
]}

Zhong, Ping ^{[1
]}

Sheng, Xiaofang ^{[3
]}

Xiao, Baoguo ^{[4
]}

Wu, Jingsong ^{[1
]}

Yang, Bojie ^{[1
]}

Mao, Ying ^{[1
]}

Zhou, Liangfu ^{[1
]}

机构：

[1] Fudan Univ, Huashan Hosp, Dept Neurosurg, Shanghai 200040, Peoples R China

[2] Fudan Univ, Shanghai Med Sch, Dept Immunol, Shanghai 200032, Peoples R China

[3] Shanghai Gamma Knife Hosp, Dept Radiotherapy, Shanghai 200235, Peoples R China

[4] Fudan Univ, Inst Neurol, Huashan Hosp, Shanghai 200040, Peoples R China

来源：

JOURNAL OF NEURO-ONCOLOGY | 2011年 / 105卷 / 02期

关键词：

Malignant gliomas; Stem-like cell-associated antigens; Dendritic cells; Immunotherapy; IDENTIFICATION; VACCINATION; EXPRESSION; TARGET; LINES;

D O I：

10.1007/s11060-011-0572-y

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

To explore the immunogenicity of glioma stem-like cell-associated antigens (SAAs) from sorted or unsorted glioma tumor stem-like cells (TSCs) as well as irradiated TSCs. Two primary human malignant glioma lines (SHG62, SHG66) and U87 cell line were primarily cultured in the serum-free medium (SFM) supplemented with EGF/bFGF. TSCs were identified by their self-renewal, multi-lineage differentiation and tumorigenic activity. To prepare SAAs in vitro, CD133+ TSCs were sorted either by magnetic cell sorting or with irradiation (6 Gy).The cytotoxicity induced by autogenous myeloid dendritic cell (DC)-mediated SAA-specific cytotoxic T lymphocytes (CTLs) was assessed by the Just Another Method test. SHG62, SHG66, and U87 cells contained TSCs. CD133+ SAAs-specific CTLs were significantly more cytotoxic than effector cells loaded with unsorted SAA (P < 0.05). Effector cells loaded with irradiated SAAs were more cyotoxic than those with regular SAAs (P < 0.01). SAAs from CD133+ TSCs and irradiated TSCs provide highly immunogenic antigens. TSCs might be a novel source of antigens for DC vaccination against malignant gliomas.

引用

页码：149 / 157

页数：9

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