Antibiotic resistance in the viral fraction of dairy products and a nut-based milk

被引:10
作者
Blanco-Picazo, Pedro [1 ]
Gomez-Gomez, Clara [1 ]
Morales-Cortes, Sara [1 ]
Muniesa, Maite [1 ]
Rodriguez-Rubio, Lorena [1 ]
机构
[1] Univ Barcelona, Dept Genet Microbiol & Stat, Diagonal 643,Annex,Floor 0, Barcelona 08028, Spain
关键词
Bacteriophage; Transduction; ARG; Horchata; BACTERIOPHAGE FRACTIONS; GENES; WASTE; BACTERIAL; PHAGE; PARTICLES; ABUNDANCE; PERSISTENCE; MICROBIOTA; VIRUSES;
D O I
10.1016/j.ijfoodmicro.2022.109590
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Phages, the most abundant biological entities in the biosphere, can carry different bacterial genes, including those conferring antibiotic resistance. In this study, dairy products were analyzed by qPCR for the presence of phages and phage particles containing antibiotic resistance genes (ARGs). Eleven ARGs were identified in 50 samples of kefir, yogurt, milk, fresh cheese and nut-based milk (horchata), purchased from local retailers in Barcelona. Propagation experiments showed that at least some of the phages isolated from these samples infected Escherichia coli WG5, which was selected as the host strain because it does not contain prophages or ARGs in its genome. Electron microscopy revealed that the phage particles showed morphologies compatible with the Myoviridae and Siphoviridae families. Our results show that dairy products contain ARGs within infectious phage particles and may therefore serve as a reservoir of ARGs that can be mobilized to susceptible hosts, both in the food matrix and in the intestinal tract after ingestion.
引用
收藏
页数:8
相关论文
共 83 条
[1]   Abundance of antibiotic resistance genes in environmental bacteriophages [J].
Anand, Taruna ;
Bera, Bidhan Ch. ;
Vaid, Rajesh K. ;
Barua, Sanjay ;
Riyesh, Thachamvally ;
Virmani, Nitin ;
Hussain, Mubarik ;
Singh, Raj K. ;
Tripathi, Bhupendra N. .
JOURNAL OF GENERAL VIROLOGY, 2016, 97 :3458-3466
[2]  
[Anonymous], 2003, Official Journal of the European Union, L, V268, P29
[3]  
[Anonymous], 2000, ISO 10705-2
[4]  
[Anonymous], 2005, OFFICIAL J EUROPEAN, V338, P1
[5]   A 100-Year Review: Yogurt and other cultured dairy products [J].
Aryana, Kayanush J. ;
Olson, Douglas W. .
JOURNAL OF DAIRY SCIENCE, 2017, 100 (12) :9987-10013
[6]   Review: elimination of bacteriophages in whey and whey products [J].
Atamer, Zeynep ;
Samtlebe, Meike ;
Neve, Horst ;
Heller, Knut J. ;
Hinrichs, Joerg .
FRONTIERS IN MICROBIOLOGY, 2013, 4
[7]   Antibiotic Resistance Genes in Phage Particles from Antarctic and Mediterranean Seawater Ecosystems [J].
Blanco-Picazo, Pedro ;
Roscales, Gabriel ;
Toribio-Avedillo, Daniel ;
Gomez-Gomez, Clara ;
Avila, Conxita ;
Balleste, Elisenda ;
Muniesa, Maite ;
Rodriguez-Rubio, Lorena .
MICROORGANISMS, 2020, 8 (09) :1-15
[8]   Detection of Bacteriophage Particles Containing Antibiotic Resistance Genes in the Sputum of Cystic Fibrosis Patients [J].
Brown-Jaque, Maryury ;
Rodriguez Oyarzun, Lirain ;
Cornejo-Sanchez, Thais ;
Martin-Gomez, Maria T. ;
Gartner, Silvia ;
de Gracia, Javier ;
Rovira, Sandra ;
Alvarez, Antonio ;
Jofre, Joan ;
Gonzalez-Lopez, Juan J. ;
Muniesa, Maite .
FRONTIERS IN MICROBIOLOGY, 2018, 9
[9]   Antibiotic resistance genes in phage particles isolated from human faeces and induced from clinical bacterial isolates [J].
Brown-Jaque, Maryury ;
Calero-Caceres, William ;
Espinal, Paula ;
Rodriguez-Navarro, Judith ;
Miro, Elisenda ;
Jose Gonzalez-Lopez, Juan ;
Cornejo, Thais ;
Carlos Hurtado, Juan ;
Navarro, Ferran ;
Muniesa, Maite .
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2018, 51 (03) :434-442
[10]   Heavy use of prophylactic antibiotics in aquaculture: a growing problem for human and animal health and for the environment [J].
Cabello, Felipe C. .
ENVIRONMENTAL MICROBIOLOGY, 2006, 8 (07) :1137-1144