The Morphology of Self-Assembled Lipid-Based Nanoparticles Affects Their Uptake by Cancer Cells

被引：31

作者：

Aresh, Wafa ^{[1
]}

Liu, Ying ^{[2
]}

Sine, Jessica ^{[3
]}

Thayer, Derek ^{[3
]}

Puri, Anu ^{[3
]}

Huang, Yike ^{[4
]}

Wang, Yong ^{[4
]}

Nieh, Mu-Ping ^{[1
,2
,5
]}

机构：

[1] Univ Connecticut, Dept Biomed Engn, Storrs, CT 06269 USA

[2] Univ Connecticut, Dept Chem & Biomol Engn, Storrs, CT 06269 USA

[3] NCI, Basic Res Lab, Ctr Canc Res, NIH, Frederick, MD 21702 USA

[4] Penn State Univ, Dept Biomed Engn, University Pk, PA 16802 USA

[5] Univ Connecticut, Inst Mat Sci, Polymer Program, Storrs, CT 06269 USA

来源：

JOURNAL OF BIOMEDICAL NANOTECHNOLOGY | 2016年 / 12卷 / 10期

关键词：

Cancer Cellular Internalization; Spontaneous Formation; Morphology-Targeting; Bicelles; Vesicles; SANS; FORMED UNILAMELLAR VESICLES; CELLULAR UPTAKE; ENGINEERED NANOPARTICLES; MAMMALIAN-CELLS; DRUG-DELIVERY; SIZE; SHAPE; LIPOSOMES; NANODISCS; INTERNALIZATION;

D O I：

10.1166/jbn.2016.2292

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

The morphology of nanoparticles (NPs) has been presumed to play an important role in cellular uptake and in vivo stability. This report experimentally demonstrates such dependence by using two types of uniform-sized self-assembled lipid-based NPs, namely nanodiscs and nanovesicles, composed of identical lipid composition. The morphology is characterized by small angle neutron scattering, dynamic light scattering and transmission electron microscopy. Both NPs have similar bio-stability in serum and cellular cytotoxicity. However, cellular uptake of the nanodiscs at 37 degrees C is consistently and significantly higher than that of the vesicles according to the uptake results of several human cancer cell lines, i.e., CCRF-CEM, KB, and OVCAR-8, indicating a strong morphological dependence of cellular internalization. Further studies on such morphological dependence using CCRF-CEM reveals that vesicles only use Clathrin-and caveolae-mediated endocytic pathways, while nanodiscs also take the additional routes of macropinocytosis and microtubule-mediated endocytosis.

引用

页码：1852 / 1863

页数：12

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