The effect of decellularized bone/bone marrow produced by high-hydrostatic pressurization on the osteogenic differentiation of mesenchymal stem cells

被引：101

作者：

Hashimoto, Yoshihide ^{[1
,2
]}

Funamoto, Seiichi ^{[1
,3
]}

Kimura, Tsuyoshi ^{[1
,4
]}

Nam, Kwangwoo ^{[1
,4
]}

Fujisato, Toshiya ^{[5
]}

Kishida, Akio ^{[1
,4
]}

机构：

[1] Tokyo Med & Dent Univ, Div Biofunct Mol, Inst Biomat & Bioengn, Chiyoda Ku, Tokyo 1010062, Japan

[2] Japan Soc Promot Sci, Chiyoda Ku, Tokyo 1028472, Japan

[3] Sapporo Med Univ, Dept Thorac & Cardiovasc Surg, Chuo Ku, Sapporo, Hokkaido 0608556, Japan

[4] Japan Sci & Technol Agcy, CREST, Tokyo 1020075, Japan

[5] Osaka Inst Technol, Fac Engn, Asahi Ku, Osaka 5358585, Japan

来源：

BIOMATERIALS | 2011年 / 32卷 / 29期

基金：

日本科学技术振兴机构; 日本学术振兴会;

关键词：

Decellularization; Extracellular matrix; Bone marrow; Scaffold; Hematopoiesis; BONE-MARROW; IN-VITRO; PERFUSION CULTURE; MATRIX; SCAFFOLDS; GROWTH; HYDROXYAPATITE; CONSTRUCTS; DEPOSITION; ADHESION;

D O I：

10.1016/j.biomaterials.2011.06.008

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Decellularized bone/bone marrow was prepared to provide a microenvironment mimicking that of the bone marrow for three-dimensional culture in vitro. Bone/bone marrows were hydrostatically pressed at 980 MPa at 30 degrees C for 10 min to dismantle the cells. Then, they were washed with EGM-2 and further treated in an 80% EtOH to remove the cell debris and lipid, respectively. After being rinsed and shaken with PBS again, treated bone/bone marrows were stained with hematoxylin and eosin (H-E) to assess the efficacy of decellularization. Cells were determined to have been completely removed through H-E staining of their sections and DNA quantification. Rat mesenchymal stem cells (rMSCs) were seeded on the decellularized bone/bone marrows and cultured for 21 days. The adhesion of rMSCs on or into decellularized bone/bone marrows was confirmed and proliferated over time in culture. The osteogenic differentiation effect of decellularized bone/bone marrows on rMSCs in the presence or absence of dexamethasone was investigated. Decellularized bone/bone marrows without dexamethasone significantly increased alkaline phosphatase (ALP) activity, indicating promoted osteogenic differentiation of rMSCs. In an animal study, when decellularized bone/bone marrows were implanted into the rat subcutaneous, no immune reaction occurred and clusters of the hematopoietic cells could be observed, suggesting the decellularized bone/bone marrows can provide a microenvironment in vivo. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：7060 / 7067

页数：8

共 45 条

[11] Matrix elasticity directs stem cell lineage specification [J].

Engler, Adam J. ;

Sen, Shamik ;

Sweeney, H. Lee ;

Discher, Dennis E. .

CELL, 2006, 126 (04) :677-689

[12] Myotubes differentiate optimally on substrates with tissue-like stiffness:: pathological implications for soft or stiff microenvironments [J].

Engler, AJ ;

Griffin, MA ;

Sen, S ;

Bönnetnann, CG ;

Sweeney, HL ;

Discher, DE .

JOURNAL OF CELL BIOLOGY, 2004, 166 (06) :877-887

[13] Proliferation and Differentiation of Goat Bone Marrow Stromal Cells in 3D Scaffolds With Tunable Hydrophilicity [J].

Escobar Ivirico, J. L. ;

Salmeron-Sanchez, M. ;

Gomez Ribelles, J. L. ;

Monleon Pradas, M. ;

Soria, J. M. ;

Gomes, M. E. ;

Reis, R. L. ;

Mano, J. F. .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 2009, 91B (01) :277-286

[14] Socializing with the neighbors: Stem cells and their niche [J].

Fuchs, E ;

Tumbar, T ;

Guasch, G .

CELL, 2004, 116 (06) :769-778

[15]

Fujisato T, 2005, CARDIOVASCULAR REGENERATION THERAPIES USING TISSUE ENGINEERING APPROACHES, P83, DOI 10.1007/4-431-27378-6_7

[16] The use of high-hydrostatic pressure treatment to decellularize blood vessels [J].

Funamoto, Seiichi ;

Nam, Kwangwoo ;

Kimura, Tsuyoshi ;

Murakoshi, Ayako ;

Hashimoto, Yoshihide ;

Niwaya, Kazuo ;

Kitamura, Soichiro ;

Fujisato, Toshiya ;

Kishida, Akio .

BIOMATERIALS, 2010, 31 (13) :3590-3595

[17] Decellularization of tissues and organs [J].

Gilbert, TW ;

Sellaro, TL ;

Badylak, SF .

BIOMATERIALS, 2006, 27 (19) :3675-3683

[18] Bone marrow genesis after subcutaneous delivery of rat osteogenic cell-seeded biodegradable scaffolds into nude mice [J].

Gomi, K ;

Kanazashi, M ;

Lickorish, D ;

Arai, T ;

Davies, JE .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2004, 71A (04) :602-607

[19] COMPARTMENTALIZATION OF A HEMATOPOIETIC GROWTH-FACTOR (GM-CSF) BY GLYCOSAMINOGLYCANS IN THE BONE-MARROW MICROENVIRONMENT [J].

GORDON, MY ;

RILEY, GP ;

WATT, SM ;

GREAVES, MF .

NATURE, 1987, 326 (6111) :403-405

[20] Three-dimensional microenvironments modulate fibroblast signaling responses [J].

Green, J. Angelo ;

Yamada, Kenneth M. .

ADVANCED DRUG DELIVERY REVIEWS, 2007, 59 (13) :1293-1298

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