MiR-378a-3p enhances adipogenesis by targeting mitogen-activated protein kinase 1

被引：43
作者：
Huang, Nunu [1 ,2 ]
Wang, Jiu [2 ]
Xie, Weidong [2 ]
Lyu, Qing [1 ,2 ]
Wu, Jiangbin [1 ,2 ]
He, Jie [2 ]
Qiu, Wei [1 ,2 ]
Xu, Naihan [2 ]
Zhang, Yaou [2 ]
机构：
[1] Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China
[2] Tsinghua Univ, Grad Sch Shenzhen, Div Life Sci, Key Lab Hlth Sci & Technol, Shenzhen 518055, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2015年 / 457卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Adipogenesis; MiR-378a-3p; MAPK1; GENE-EXPRESSION; RECEPTOR-GAMMA; MAP KINASE; MICRORNAS; DIFFERENTIATION; CELLS; FIBROBLASTS; ADIPOCYTES; METABOLISM; MECHANISM;
D O I：
10.1016/j.bbrc.2014.12.055
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Previous studies showed that miR-378a plays important roles in adipogenesis and obesity; however, the precise mechanisms of action remain unknown. Here, we found that miR-378a-3p expression is up-regulated in adipose tissues of high fat diet-induced obese mice, as well as during the differentiation of 3T3-L1 preadipocytes. Mir-378a-3p induced adipogenesis by targeting mitogen-activated protein kinase 1 (MAPK1). Overexpression of miR-378a-3p or silencing MAPK1 reduced MAPK1 expression and enhanced adipogenesis, whereas blockage of endogenous miR-378a-3p had the opposite effect, suggesting that miR-378a-3p promotes the adipogenesis of 3T3-L1 cells by targeting MAPK1. (C) 2014 Elsevier Inc. All rights reserved.
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收藏
页码：37 / 42
页数：6
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