Electrochemical indirect competitive immunoassay for ultrasensitive detection of zearalenone based on a glassy carbon electrode modified with carboxylated multi-walled carbon nanotubes and chitosan

被引:75
|
作者
Xu, Wei [1 ]
Qing, Ying [1 ]
Chen, Shuai [1 ]
Chen, Jun [1 ]
Qin, Zhen [1 ]
Qiu, JingFu [1 ]
Li, ChaoRui [1 ]
机构
[1] Chongqing Med Univ, Sch Publ Hlth & Management, Chongqing 400016, Peoples R China
基金
中国国家自然科学基金;
关键词
DPV; Cereal grains; Fodder; Alkaline phosphatase; alpha-Naphthyl phosphate; Electrochemical label; IMMUNOSENSOR; FABRICATION; DENDRIMER; BIOSENSOR; TOXICITY;
D O I
10.1007/s00604-017-2342-9
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The authors describe an electrochemical immunoassay for ultrasensitive detection of the mycotoxin zearalenone (ZEA). A nanocomposite was prepared from carboxy-functionalized multi-walled carbon nanotubes and chitosan (cMWCNTs/Chit). The morphology and electrochemical performance of the materials was characterized by field-emission scanning electron microscopy, atomic force microscopy, differential pulse voltammetry, cyclic voltammetry, and electrochemical impedance spectroscopy. In this assay, ZEA-BSA conjugated covalently to activated cMWCNTs/Chit film, then the indirect competition between ZEA-BSA and free ZEA when immobilization of excess anti-ZEA. The secondary antibody is labeled with the enzyme alkaline phosphatase which can hydrolyze the substrate 1-naphthylphosphate to produce 1-naphthol which gives a stable and strong anodic electrochemical signal at a low working voltage of 0.3 V (vs. Ag/AgCl). The use of the modified GCE results in a strongly enhanced electrochemical current response. Compared with conventional methods, the established immunosensor exhibited a high level of sensitivity. Under optimal conditions, this immunoassay can quantify ZEA in the 10 pg.mL-1 to 1000 ng.mL-1 concentration range with a detection limit of 4.7 pg.mL-1 and the sensitivity is 0.51 mu A.mu M-1.cm-2. The method was applied to the determination of ZEA in cereal and feedstuff samples. Results showed satisfactory recovery and good consistency with high-performance liquid chromatography. Therefore, the indirect competitive electrochemical immunosensor provide a viable tool based on bioanalysis.
引用
收藏
页码:3339 / 3347
页数:9
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