Molecular diversity of sulfate-reducing bacteria from two different continental margin habitats

被引:45
作者
Liu, XD
Bagwell, CE
Wu, LY
Devol, AH
Zhou, JH
机构
[1] Oak Ridge Natl Lab, Div Environm Sci, Oak Ridge, TN 37831 USA
[2] Hunan Agr Univ, Dept Plant Pathol, Changsha, Hunan, Peoples R China
[3] Univ Washington, Sch Oceanog, Seattle, WA 98195 USA
关键词
D O I
10.1128/AEM.69.10.6073-6081.2003
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
This study examined the natural diversity and distributions of sulfate-reducing bacteria along a natural carbon gradient extending down the shelf-slope transition zone of the eastern Pacific continental margin. Dissimilatory (bi)sulfite reductase gene sequences (dsrAB) were PCR amplified and cloned from five different sampling sites, each at a discrete depth, from two different margin systems, one off the Pacific coast of Mexico and another off the coast of Washington State. A total of 1,762 clones were recovered and evaluated by restriction fragment length polymorphism (RFLP) analysis. The majority of the gene sequences recovered showed site and depth restricted distributions; however, a limited number of gene sequences were widely distributed within and between the margin systems. Cluster analysis identified 175 unique RFLP patterns, and nucleotide sequences were determined for corresponding clones. Several different continental margin DsrA sequences clustered with those from formally characterized taxa belonging to the delta subdivision of the class Proteobacteria (Desulfobulbus propionicus, Desulfosarcina variabilis) and the Bacillus-Clostridium (Desulfotomaculum putei) divisions, although the majority of the recovered sequences were phylogenetically divergent relative to all of the other DsrA sequences available for comparison. This study revealed extensive new genetic diversity among sulfate-reducing bacteria in continental margin sedimentary habitats, which appears to be tightly coupled to slope depth, specifically carbon bioavailability.
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页码:6073 / 6081
页数:9
相关论文
共 57 条
[31]  
JORGENSEN BB, 1983, MAJOR BIOGEOCHEMICAL, V21
[32]   CONSERVATION OF THE GENES FOR DISSIMILATORY SULFITE REDUCTASE FROM DESULFOVIBRIO-VULGARIS AND ARCHAEOGLOBUS-FULGIDUS ALLOWS THEIR DETECTION BY PCR [J].
KARKHOFFSCHWEIZER, RR ;
HUBER, DPW ;
VOORDOUW, G .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (01) :290-296
[33]   Multiple lateral transfers of dissimilatory sulfite reductase genes between major lineages of sulfate-reducing prokaryotes [J].
Klein, M ;
Friedrich, M ;
Roger, AJ ;
Hugenholtz, P ;
Fishbain, S ;
Abicht, H ;
Blackall, LL ;
Stahl, DA ;
Wagner, M .
JOURNAL OF BACTERIOLOGY, 2001, 183 (20) :6028-6035
[34]   Aerobic and anaerobic decomposition of organic matter in marine sediment: Which is fastest? [J].
Kristensen, E ;
Ahmed, SI ;
Devol, AH .
LIMNOLOGY AND OCEANOGRAPHY, 1995, 40 (08) :1430-1437
[35]   Seasonal changes in ribosomal RNA of sulfate-reducing bacteria and sulfate reducing activity in a freshwater lake sediment [J].
Li, JH ;
Purdy, KJ ;
Takii, S ;
Hayashi, H .
FEMS MICROBIOLOGY ECOLOGY, 1999, 28 (01) :31-39
[36]   INFLUENCE OF OXYGEN ON SULFATE REDUCTION AND GROWTH OF SULFATE-REDUCING BACTERIA [J].
MARSCHALL, C ;
FRENZEL, P ;
CYPIONKA, H .
ARCHIVES OF MICROBIOLOGY, 1993, 159 (02) :168-173
[37]  
Minz D, 1999, APPL ENVIRON MICROB, V65, P4666
[38]  
Minz D, 1999, APPL ENVIRON MICROB, V65, P4659
[39]   Increased marine production of N2O due to intensifying anoxia on the Indian continental shelf [J].
Naqvi, SWA ;
Jayakumar, DA ;
Narvekar, PV ;
Naik, H ;
Sarma, VVSS ;
D'Souza, W ;
Joseph, S ;
George, MD .
NATURE, 2000, 408 (6810) :346-349
[40]  
Premuzic E.T., 1982, Organic Geochemistry, V4, P63, DOI [10.1016/0146-6380(82)90009-2, DOI 10.1016/0146-6380(82)90009-2]