PHYSICAL MAPPING AND INHERITANCE STUDIES OF CRY1AC GENE ON TRANSGENIC COTTON CHROMOSOMES

被引：0

作者：

Noreen, S. ^{[1
]}

Mahmood-ur-Rahman ^{[2
]}

Husanin, T. ^{[3
]}

机构：

[1] Univ Sargodha, Dept Chem, Main Campus, Sargodha, Pakistan

[2] GC Univ Faisalabad, Dept Bioinformat & Biotechnol, Allama Iqbal Rd, Faisalabad 38000, Pakistan

[3] Natl Ctr Excellence Mol Biol, 87 West Canal Bank Rd, Lahore 53700, Pakistan

来源：

JOURNAL OF ANIMAL AND PLANT SCIENCES-JAPS | 2021年 / 31卷 / 05期

关键词：

Karyotyping; Fluorescence in situ hybridization; transgene; cry1Ac; physical mapping; IN-SITU HYBRIDIZATION; GENOME; PLANTS; LOCALIZATION; EXPRESSION; CHROMATIN; BARLEY; WHEAT;

D O I：

10.36899/JAPS.2021.5.0348

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

The presence of Bt gene, cry1Ac in transgenic lines of Gossypium hirsutum L. was determined by molecular and cytogenetic analysis. Fluorescence in situ hybridization (FISH) was carried out to study the location of transgene on chromosomes. Metaphase chromosomes prepared from root tips and pollen grains showed more condensation, and hence more karyotypically differentiated. The five transgenic lines of cotton showed stable integration of transgene, cry1Ac. Either the lines were heterozygous (CEMB-3010-14, CEMB-3013-10, CEMB-3016-13 and CEMB-3037-2) or homozygous (CEMB-3034-10) showing single and double genes respectively at interphase and metaphase FISH while the control lines showed no fluorescent signal for transgene at all. Metaphase chromosomes prepared from pollens were also analyzed and single copy of cry1Ac was mapped on chromosome 10 which showed the stable inheritance of transgene in the progeny. The information obtained during study may be helpful to understand the possible effects of copy number variation and position effect of transgene in Bt cotton. The study may also help to comprehend the process of transgene inheritance in Bt cotton lines.

引用

页码：1470 / 1476

页数：7