In vitro osteogenic differentiation of HOS cells on two types of collagen gels

被引：12

作者：

Takitoh, Takako ^{[1
]}

Kato, Yoichi ^{[2
]}

Nakasu, Asako ^{[2
]}

Tadokoro, Mika ^{[2
]}

Bessho, Masahiko ^{[1
]}

Hirose, Motohiro ^{[2
]}

Ohgushi, Hajime ^{[2
]}

Mori, Hideki ^{[1
]}

Hara, Masayuki ^{[1
]}

机构：

[1] Osaka Prefecture Univ, Grad Sch Sci, Dept Biol Sci, Naka Ku, Osaka 5998570, Japan

[2] AIST, Res Inst Cell Engn, Amagasaki, Hyogo 65610974, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2010年 / 110卷 / 04期

关键词：

HOS cell; Osteoblast; Osteogenesis; Calcium; Collagen; Collagen febril; HUMAN OSTEOSARCOMA; MINERALIZATION; ALPHA-2-BETA-1;

D O I：

10.1016/j.jbiosc.2010.04.009

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

HOS cell is a model strain of human osteoblasts derived from human osteosarcoma. We cultured the HOS cells on both the conventional collagen gel (neutral gel), and the gamma-crosslinked collagen gel without collagen fibrils (acidic gel). The shape of HOS cells on the neutral gel was similar to that on the culture dish. However. HOS cells on acidic gel had an elongated shape and attached each other to form a mesh-like pattern. The cells attached to the surface of both gels but scarcely penetrated their depths. We measured the biochemical markers for osteogenic differentiation in the HOS cells cultured on both the neutral gel and the acidic gel. The expressions of alkaline phosphatase and osteocalcin were detected in the HOS cells on both types of collagen gel. Deposition of the calcium also occurred on both gels although it was higher in the neutral gel than the acidic one. These results indicate the importance of collagen for the differentiation of HOS cells, but it is not dependent on the molecular structure (fibril formation) of collagen. (C) 2010, The Society for Biotechnology, Japan. All rights reserved.

引用

页码：471 / 478

页数：8

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