Efficient whole-cell biocatalytic synthesis of N-acetyl-D-neuraminic acid

被引：34

作者：

Xu, Ping ^{[1
]}

Qiu, Jian Hua

Zhang, Yi Nan

Chen, Jing

Wang, Peng George

Yan, Bing

Song, Jing

Xi, Ri Mo

Deng, Zi Xin

Ma, Cui Qing

机构：

[1] Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Shandong Prov, Peoples R China

[2] Shanghai Jiao Tong Univ, Coll Life Sci & Biotechnol, Minist Educ, Key Lab Microbial Metab, Shanghai 200240, Peoples R China

[3] Shandong Univ, Sch Pharmaceut Sci, Jinan 250100, Shandong Prov, Peoples R China

[4] Shandong Univ, Dept Chem, Jinan 250100, Shandong Prov, Peoples R China

来源：

ADVANCED SYNTHESIS & CATALYSIS | 2007年 / 349卷 / 10期

关键词：

n-acetyl-D-neuraminic acid (Neu5Ac); biotransformation; lactate oxidase component; Neu5Ac aldolase; sialic acids; whole cells;

D O I：

10.1002/adsc.200700094

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

N-Acetyl-D-neuraminic acid (Neu5Ac) was efficiently synthesized from lactate and a mixture of N-acetyl-D-glucosamine (GlcNAc) and N-acetyl-D-mannosamine (ManNAc) by whole cells. The biotransformation utilized Escherichia coli cells (Neu5Ac aldolase), Pseudomonas stutzeri cells (lactate oxidase components), GlcNAc/ManNAc and lactate. By this process, 18.32 +/- 0.56 g/liter Neu5Ac were obtained from 65.61 +/- 2.70 g/liter lactate as an initial substrate input. Neu5Ac (98.4 +/- 0.4% purity, 80.87 +/- 0.79% recovery yield) was purified by anionic exchange chromatography. Our results demonstrate that the reported Neu5Ac biosynthetic process can compare favorably with natural product extraction or chemical synthesis processes.

引用

页码：1614 / 1618

页数：5

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