Microbial assisted High Impact Polystyrene (HIPS) degradation

被引:148
作者
Mohan, Arya J. [1 ]
Sekhar, Vini C. [1 ]
Bhaskar, Thallada [2 ]
Nampoothiri, K. Madhavan [1 ]
机构
[1] CSIR, NIIST, Div Biotechnol, Trivandrum 695019, Kerala, India
[2] Indian Inst Petr, CSIR, Biofuels Div, Dehra Dun 248005, Uttarakhand, India
关键词
High Impact Polystyrene; Pseudomonas species; Bacillus species; Methyl bromine; BIODEGRADATION;
D O I
10.1016/j.biortech.2016.03.021
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
The efficacy of newly isolated Pseudomonas and Bacillus strains to degrade brominated High Impact Polystyrene (HIPS) was investigated. Viability of these cultures while using e-plastic as sole carbon source was validated through Triphenyl Tetrazolium Chloride (TTC). Four days incubation of HIPS emulsion with Bacillus spp. showed 94% reduction in turbidity and was 97% with Pseudomonas spp. Confirmation of degradation was concluded by HPLC, NMR, FTIR, TGA and weight loss analysis. NMR spectra of the degraded film revealed the formation of aliphatic carbon chain with bromine and its release. FTIR analysis of the samples showed a reduction in CAH, C=O C=N groups. Surface changes in the brominated HIPS film was visualized through SEM analysis. Degradation with Bacillus spp. showed a weight loss of 23% (w/w) of HIPS film in 30 days. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码:204 / 207
页数:4
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