Derivation and maintenance of human embryonic stem cells from poor-quality in vitro fertilization embryos

被引：34

作者：

Lerou, Paul H. ^{[2
]}

Yabuuchi, Akiko ^{[1
]}

Huo, Hongguang ^{[1
,3
]}

Miller, Justine D. ^{[1
,3
]}

Boyer, Leah F. ^{[1
,3
]}

Schlaeger, Thorsten M. ^{[1
,3
]}

Daley, George Q. ^{[1
,3
,4
,5
,6
,7
]}

机构：

[1] Childrens Hosp, Div Pediat Hematol Oncol, Boston, MA 02115 USA

[2] Brigham & Womens Hosp, Div Newborn Med, Boston, MA 02115 USA

[3] Childrens Hosp, hES Cell Facil, Harvard Stem Cell Inst, Boston, MA 02115 USA

[4] Brigham & Womens Hosp, Div Hematol, Boston, MA 02115 USA

[5] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA

[6] Harvard Stem Cell Inst, Boston, MA 02115 USA

[7] Howard Hughes Med Inst, Boston, MA 02115 USA

来源：

NATURE PROTOCOLS | 2008年 / 3卷 / 05期

关键词：

D O I：

10.1038/nprot.2008.60

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Human embryonic stem (hES) cells are self-renewing, pluripotent cells that are valuable research tools and hold promise for use in regenerative medicine. Most hES cell lines are derived from cryopreserved human embryos that were created during in vitro fertilization (IVF) and are in excess of clinical need. Embryos that are discarded during the IVF procedure because of poor morphology and a low likelihood for generating viable pregnancies or surviving the cryopreservation process are also a viable source of hES cells. In this protocol, we describe how to derive novel hES cells from discarded poor-quality embryos and how to maintain the hES cell lines.

引用

页码：923 / 933

页数：11

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