Evaluation of cell behavior on modified polypropylene with swift heavy ion irradiation

被引：1

作者：

Arbeitman, Claudia R. ^{[1
,2
]}

Ibanez, Irene L. ^{[2
]}

Garcia Bermudez, Gerardo ^{[1
,2
]}

Duran, Hebe ^{[1
,2
]}

del Grosso, Mariela F. ^{[1
,2
]}

Salguero, Noelia ^{[1
]}

Mazzei, Ruben

机构：

[1] TANDAR CNEA, RA-1650 Buenos Aires, DF, Argentina

[2] Consejo Nacl Invest Cient & Tecn, CABA, RA-1033 Buenos Aires, DF, Argentina

来源：

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS | 2012年 / 273卷

关键词：

Cell adhesion; Biocompatibility; Irradiated polymers; Swift heavy ions; ADHESION; RESPONSES; POLYMERS;

D O I：

10.1016/j.nimb.2011.07.036

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

Ion beam irradiation is a well known means to change the physico-chemical properties of polymers, and induced bio and citocompatibility in controlled conditions and in selected areas of surface. However, the enhancement of cell adhesion on a modified substrate does not mean that the surface is adequate for functional cells. The purpose of the present work is to study proliferation, changes in cytoskeleton and cell morphology on substrates as a function of irradiation parameters. We irradiated polypropylene with sulfur (S) ion-beam at energies of 110 MeV with fluences between 1 x 10(6) and 2 x 10(10) ions cm(-2). NIH 3T3 cells were cultured on each sample. Cell morphology was observed using phase contrast microscopy and cytoskeleton proteins with fluorescence microscopy. The analysis show different cellular responses as a functions of irradiation parameter, strongly suggests that different underlying substratum can result in distinct types of cytoskeleton reorganization. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：51 / 54

页数：4

共 19 条

[1] Interaction of soft condensed materials with living cells: Phenotype/transcriptome correlations for the hydrophobic effect [J].

Allen, LT ;

Fox, EJP ;

Blute, I ;

Kelly, ZD ;

Rochev, Y ;

Keenan, AK ;

Dawson, KA ;

Gallagher, WM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (11) :6331-6336

[2] Biological responses to materials [J].

Anderson, JM .

ANNUAL REVIEW OF MATERIALS RESEARCH, 2001, 31 :81-110

[3]

[Anonymous], 1990, Ion Tracks and Microtechnology, Principles and Applications

[4] Irradiation of Polystyrene and Polypropylene to study NIH 3T3 fibroblasts adhesion [J].

Arbeitman, C. R. ;

del Grosso, M. F. ;

Ibanez, I. ;

Garcia Bermudez, G. ;

Duran, H. ;

Chappa, V. C. ;

Mazzei, R. ;

Behar, M. .

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS, 2010, 268 (19) :3059-3062

[5]

Backov L., 2000, BIOMED MAT RES, V49, P369

[6] Focal adhesions, contractility, and signaling [J].

Burridge, K ;

ChrzanowskaWodnicka, M .

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 1996, 12 :463-518

[7] Geometric control of cell life and death [J].

Chen, CS ;

Mrksich, M ;

Huang, S ;

Whitesides, GM ;

Ingber, DE .

SCIENCE, 1997, 276 (5317) :1425-1428

[8] Plasma treatment of polymers [J].

Grace, JM ;

Gerenser, LJ .

JOURNAL OF DISPERSION SCIENCE AND TECHNOLOGY, 2003, 24 (3-4) :305-341

[9] Anchorage-dependent ERK signaling - mechanisms and consequences [J].

Howe, AK ;

Aplin, AE ;

Juliano, RL .

CURRENT OPINION IN GENETICS & DEVELOPMENT, 2002, 12 (01) :30-35

[10] ADHESION IN CELL-MIGRATION [J].

HUTTENLOCHER, A ;

SANDBORG, RR ;

HORWITZ, AF .

CURRENT OPINION IN CELL BIOLOGY, 1995, 7 (05) :697-706

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