EBV-encoded LMP1 increases nuclear β-catenin accumulation and its transcriptional activity in nasopharyngeal carcinoma

被引：15

作者：

You, Shuyuan ^{[1
,2
,3
]}

Zhang, Fenfen ^{[1
,2
]}

Meng, Fengjiao ^{[1
,2
]}

Li, Hui ^{[1
,2
]}

Liu, Qiuyu ^{[4
]}

Liang, Yingjie ^{[1
,2
]}

Dong, Yu ^{[1
,2
]}

Yang, Wancai ^{[5
]}

Han, Anjia ^{[1
,2
]}

机构：

[1] Sun Yat Sen Univ, Dept Pathol, Affiliated Hosp 1, Guangzhou 510080, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, Zhongshan Sch Med, Guangzhou 510080, Guangdong, Peoples R China

[3] Peoples Hosp Yantian Dist, Dept Pathol, Shenzhen 518081, Peoples R China

[4] Peoples Hosp Henan Prov, Dept Pathol, Zhengzhou 450000, Peoples R China

[5] Univ Illinois, Dept Pathol, Chicago, IL 60612 USA

来源：

TUMOR BIOLOGY | 2011年 / 32卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Nasopharyngeal carcinoma; LMP1; beta-catenin; EPSTEIN-BARR-VIRUS; LATENT MEMBRANE PROTEIN-1; LYMPHOCYTE GROWTH TRANSFORMATION; COLORECTAL-CANCER; SIGNALING PATHWAY; EPITHELIAL-CELLS; EXPRESSION; MATRIX-METALLOPROTEINASE-9; METASTASIS; INDUCTION;

D O I：

10.1007/s13277-011-0161-x

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

This paper aimed to study whether Epstein-Barr virus-encoded latent membrane protein 1 (LMP1) regulates beta-catenin signaling pathway in nasopharyngeal carcinoma (NPC). Western blotting, immunofluorescence, luciferase reporter assay, co-immunoprecipitation assay, and immunohistochemistry staining were used. LMP1 increased beta-catenin transcriptional activity in NPC cell lines. The upregulation of beta-catenin transcriptional activity induced by LMP1 was much higher in poorly differentiated NPC cell line CNE2 than that in well-differentiated NPC cell line CNE1. Immunofluorescence staining and Western blotting also showed that LMP1 increased nuclear beta-catenin accumulation in NPC cell lines. Moreover, LMP1 expression was closely related to abnormal beta-catenin expression in NPC tissues by immunohistochemistry. LMP1 may be involved in nasopharyngeal carcinogenesis via beta-catenin signaling pathway.

引用

页码：623 / 630

页数：8

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