Structural and Mechanical Properties of Klebsiella pneumoniae Type 3 Fimbriae

被引：19

作者：

Chen, Feng-Jung ^{[1
,2
,3
,4
]}

Chan, Chia-Han ^{[1
,2
]}

Huang, Ying-Jung ^{[5
]}

Liu, Kuo-Liang ^{[6
]}

Peng, Hwei-Ling ^{[5
]}

Chang, Hwan-You ^{[7
,8
]}

Liou, Gunn-Guang ^{[10
]}

Yew, Tri-Rung ^{[6
]}

Liu, Cheng-Hsien ^{[9
]}

Hsu, Ken Y. ^{[3
,4
]}

Hsu, Long ^{[1
,2
]}

机构：

[1] Natl Chiao Tung Univ, Inst Electrophys, Hsinchu 300, Taiwan

[2] Natl Chiao Tung Univ, Dept Electrophys, Hsinchu 300, Taiwan

[3] Natl Chiao Tung Univ, Dept Photon, Hsinchu 300, Taiwan

[4] Natl Chiao Tung Univ, Inst Electroopt Engn, Hsinchu 300, Taiwan

[5] Natl Chiao Tung Univ, Dept Biol Sci & Technol, Hsinchu 300, Taiwan

[6] Natl Tsing Hua Univ, Dept Mat Sci & Engn, Hsinchu 300, Taiwan

[7] Natl Tsing Hua Univ, Inst Mol Med, Hsinchu 300, Taiwan

[8] Natl Tsing Hua Univ, Dept Life Sci, Hsinchu 300, Taiwan

[9] Natl Tsing Hua Univ, Dept Power Mech Engn, Hsinchu 300, Taiwan

[10] Natl Hlth Res Inst, Div Mol & Genom Med, Jhunan 350, Miaoli County, Taiwan

来源：

JOURNAL OF BACTERIOLOGY | 2011年 / 193卷 / 07期

关键词：

UROPATHOGENIC ESCHERICHIA-COLI; BACTERIAL ADHESION PILI; OPTICAL TWEEZERS; P-PILI; BIOMECHANICAL PROPERTIES; NUCLEOTIDE-SEQUENCE; FORCE MICROSCOPY; STICKY CHAIN; ADHERENCE; DNA;

D O I：

10.1128/JB.01395-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

This study investigated the structural and mechanical properties of Klebsiella pneumoniae type 3 fimbriae, which constitute a known virulence factor for the bacterium. Transmission electron microscopy and optical tweezers were used to understand the ability of the bacterium to survive flushes. An individual K. pneumoniae type 3 fimbria exhibited a helix-like structure with a pitch of 4.1 nm and a three-phase force-extension curve. The fimbria was first nonlinearly stretched with increasing force. Then, it started to uncoil and extended several micrometers at a fixed force of 66 +/- 4 pN (n +/- 22). Finally, the extension of the fimbria shifted to the third phase, with a characteristic force of 102 +/- 9 pN (n = 14) at the inflection point. Compared with the P fimbriae and type 1 fimbriae of uropathogenic Escherichia coli, K. pneumoniae type 3 fimbriae have a larger pitch in the helix-like structure and stronger uncoiling and characteristic forces.

引用

页码：1718 / 1725

页数：8

共 45 条

[1] A sticky chain model of the elongation and unfolding of Escherichia coli P pili under stress [J].