New Toxicity Mechanism of Silver Nanoparticles: Promoting Apoptosis and Inhibiting Proliferation

被引：64

作者：

Bao, Huijing ^{[1
]}

Yu, Xiaoxu ^{[1
]}

Xu, Chen ^{[2
]}

Li, Xue ^{[1
]}

Li, Zhaoyang ^{[3
]}

Wei, Dianjun ^{[4
]}

Liu, Yunde ^{[1
]}

机构：

[1] Tianjin Med Univ, Sch Lab Sci, Tianjin, Peoples R China

[2] 4th Cent Hosp, Tianjin, Peoples R China

[3] Tianjin Univ, Sch Mat Sci & Engn, Tianjin 300072, Peoples R China

[4] Tianjin Med Univ, Hosp 2, Dept Lab Sci, Tianjin, Peoples R China

来源：

PLOS ONE | 2015年 / 10卷 / 03期

关键词：

ANTIBACTERIAL ACTIVITY; CELL-DEATH; SURFACE;

D O I：

10.1371/journal.pone.0122535

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Silver nanoparticles are increasingly recognized for their utility in biological applications, especially antibacterial effects. Herein, we confirmed the antibacterial effect of silver nanoparticles on Escherichia coli using the conventional optical density (OD) and colony-forming units (CFU) method and used flow cytometry (FC), TEM and BrdU ELISA to investigate the mechanisms of this effect. From the results, we conclude that AgNPs can simultaneously induce apoptosis and inhibit new DNA synthesis in the cells in a positive concentration-dependent manner. This study presents the first induction of apoptosis in these bacteria by AgNPs in this field. Our findings may provide a new strategy for the use of silver nanoparticles in antibacterial applications.

引用

页数：10

共 23 条

[1] Antimicrobial activity and the mechanism of silver nanoparticle thermosensitive gel [J].

Chen, Meiwan ;

Yang, Zhiwen ;

Wu, Hongmei ;

Pan, Xin ;

Xie, Xiaobao ;

Wu, Chuanbin .

INTERNATIONAL JOURNAL OF NANOMEDICINE, 2011, 6 :2873-2877

[2] Nitrification inhibition by silver nanoparticles [J].

Choi, O. K. ;

Hu, Z. Q. .

WATER SCIENCE AND TECHNOLOGY, 2009, 59 (09) :1699-1702

[3] In Situ Study of the Antibacterial Activity and Mechanism of Action of Silver Nanoparticles by Surface-Enhanced Raman Spectroscopy [J].

Cui, Li ;

Chen, Pengyu ;

Chen, Shaode ;

Yuan, Zhihua ;

Yu, Changping ;

Ren, Bin ;

Zhang, Kaisong .

ANALYTICAL CHEMISTRY, 2013, 85 (11) :5436-5443

[4] YihE Kinase Is a Central Regulator of Programmed Cell Death in Bacteria [J].

Dorsey-Oresto, Angella ;

Lu, Tao ;

Mosel, Michael ;

Wang, Xiuhong ;

Salz, Tal ;

Drlica, Karl ;

Zhao, Xilin .

CELL REPORTS, 2013, 3 (02) :528-537

[5]

Dwyer Daniel J, 2013, Methods Mol Biol, V1004, P145, DOI 10.1007/978-1-62703-383-1_11

[6] Antibiotic-Induced Bacterial Cell Death Exhibits Physiological and Biochemical Hallmarks of Apoptosis [J].

Dwyer, Daniel J. ;

Camacho, Diogo M. ;

Kohanski, Michael A. ;

Callura, Jarred M. ;

Collins, James J. .

MOLECULAR CELL, 2012, 46 (05) :561-572

[7] Two Programmed Cell Death Systems in Escherichia coli: An Apoptotic-Like Death Is Inhibited by the mazEF-Mediated Death Pathway [J].

Erental, Ariel ;

Sharon, Idith ;

Engelberg-Kulka, Hanna .

PLOS BIOLOGY, 2012, 10 (03)

[8]

FADOK VA, 1992, J IMMUNOL, V148, P2207

[9] Silver Coordination Polymers for Prevention of Implant Infection: Thiol Interaction, Impact on Respiratory Chain Enzymes, and Hydroxyl Radical Induction [J].

Gordon, Oliver ;

Slenters, Tunde Vig ;

Brunetto, Priscilla S. ;

Villaruz, Amer E. ;

Sturdevant, Daniel E. ;

Otto, Michael ;

Landmann, Regine ;

Fromm, Katharina M. .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2010, 54 (10) :4208-4218

[10] Antibiotic development challenges: the various mechanisms of action of antimicrobial peptides and of bacterial resistance [J].

Guilhelmelli, Fernanda ;

Vilela, Nathalia ;

Albuquerque, Patricia ;

Derengowski, Lorena da S. ;

Silva-Pereira, Ildinete ;

Kyaw, Cynthia M. .

FRONTIERS IN MICROBIOLOGY, 2013, 4

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