New Toxicity Mechanism of Silver Nanoparticles: Promoting Apoptosis and Inhibiting Proliferation

被引：61

作者：

Bao, Huijing ^{[1
]}

Yu, Xiaoxu ^{[1
]}

Xu, Chen ^{[2
]}

Li, Xue ^{[1
]}

Li, Zhaoyang ^{[3
]}

Wei, Dianjun ^{[4
]}

Liu, Yunde ^{[1
]}

机构：

[1] Tianjin Med Univ, Sch Lab Sci, Tianjin, Peoples R China

[2] 4th Cent Hosp, Tianjin, Peoples R China

[3] Tianjin Univ, Sch Mat Sci & Engn, Tianjin 300072, Peoples R China

[4] Tianjin Med Univ, Hosp 2, Dept Lab Sci, Tianjin, Peoples R China

来源：

PLOS ONE | 2015年 / 10卷 / 03期

关键词：

ANTIBACTERIAL ACTIVITY; CELL-DEATH; SURFACE;

D O I：

10.1371/journal.pone.0122535

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Silver nanoparticles are increasingly recognized for their utility in biological applications, especially antibacterial effects. Herein, we confirmed the antibacterial effect of silver nanoparticles on Escherichia coli using the conventional optical density (OD) and colony-forming units (CFU) method and used flow cytometry (FC), TEM and BrdU ELISA to investigate the mechanisms of this effect. From the results, we conclude that AgNPs can simultaneously induce apoptosis and inhibit new DNA synthesis in the cells in a positive concentration-dependent manner. This study presents the first induction of apoptosis in these bacteria by AgNPs in this field. Our findings may provide a new strategy for the use of silver nanoparticles in antibacterial applications.

引用

页数：10

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