The role of the reaction medium in lipase-catalyzed esterifications and transesterifications

被引：51

作者：

Cernia, E ^{[1
]}

Palocci, C ^{[1
]}

Soro, S ^{[1
]}

机构：

[1] Univ Rome La Sapienza, Dept Chem, I-00185 Rome, Italy

来源：

CHEMISTRY AND PHYSICS OF LIPIDS | 1998年 / 93卷 / 1-2期

关键词：

lipase; biocatalysis; organic solvent; supercritical fluids;

D O I：

10.1016/S0009-3084(98)00040-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The nature of the reaction medium (different organic solvents and supercritical fluids) markedly influenced the microbial lipase activity in esterification and transesterification reactions employing as substrates natural and synthetic compounds in terms of reaction rates and lipase enantioselectivity. The experimental data obtained show that while there are no substantial correlations between enantioselectivity and some physicochemical characteristics of the solvent as hydrophobicity and dielectric constant, the solvent polarity and hydrophobicity is able to modulate greatly lipase activity. The possible effects of the solvent characteristics on the catalytic performance of the enzymes are discussed and a rationale is proposed to explain the results obtained. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：157 / 168

页数：12

共 27 条

[1] ENZYMATIC CATALYSIS AND DYNAMICS IN LOW-WATER ENVIRONMENTS [J].

AFFLECK, R ;

XU, ZF ;

SUZAWA, V ;

FOCHT, K ;

CLARK, DS ;

DORDICK, JS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (03) :1100-1104

[2] TOTAL SYNTHESIS OF BREFELDIN-A [J].

BARTLETT, PA ;

GREEN, FR .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1978, 100 (15) :4858-4865

[3]

BAUDOUY R, 1977, Tetrahedron Letters, V34, P2973

[4]

Bauer K., 1990, COMMON FRAGRANCE FLA

[5] IMMUNIZATION OF LIPASE AGAINST ACETALDEHYDE EMERGING IN ACYL TRANSFER-REACTIONS FROM VINYL-ACETATE [J].

BERGER, B ;

FABER, K .

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1991, (17) :1198-1200

[6] IDENTITY OF CYANEIN AND BREFELDIN A [J].

BETINA, V ;

NEMEC, P ;

KOVAC, S ;

KJAER, A ;

SHAPIRO, RH .

ACTA CHEMICA SCANDINAVICA, 1965, 19 (02) :519-&

[7]

BETINA V, 1969, NEOPLASMA, V16, P23

[8] THE FUTURE-IMPACT OF INDUSTRIAL LIPASES [J].

BJORKLING, F ;

GODTFREDSEN, SE ;

KIRK, O .

TRENDS IN BIOTECHNOLOGY, 1991, 9 (10) :360-363

[9]

CATONI E, 1996, J MOL CATAL, P105

[10]

Cernia E, 1997, METHOD ENZYMOL, V286, P495

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