An experimental study on intracerebroventricular transplantation of human amniotic epithelial cells in a rat model of Parkinson's disease

被引：26
作者：
Yang, Xinxin [2 ]
Song, Lu [2 ]
Wu, Na [2 ]
Liu, Zhenguo [2 ]
Xue, Shouru [1 ]
Hui, Guozhen [3 ]
机构：
[1] Soochow Univ, Affiliated Hosp 1, Dept Neurol, Suzhou, Peoples R China
[2] Shanghai Jiao Tong Univ, Sch Med, Xinhua Hosp, Shanghai 200030, Peoples R China
[3] Soochow Univ, Affiliated Hosp 1, Dept Neurosurg, Suzhou, Peoples R China
来源：
NEUROLOGICAL RESEARCH | 2010年 / 32卷 / 10期
基金：
中国国家自然科学基金;
关键词：
Human amniotic epithelial cells; Parkinson's disease; Transplantation; Dopamine; ABNORMAL INVOLUNTARY MOVEMENTS; EMBRYONIC STEM-CELLS; DOPAMINERGIC-NEURONS; L-DOPA; EXPRESSION; DYSKINESIA; IMPROVES; MARKERS;
D O I：
10.1179/016164110X12681290831207
中图分类号：
R74 [神经病学与精神病学];
学科分类号：
摘要：
Objectives: Human amniotic epithelial (HAE) cells are formed from amnioblasts, separated from the epiblast at about the eighth day after fertilization. In the present study, we attempt to investigate the effects of intracerebroventricular transplantation of HAE cells on Parkinson's disease (PD) rats. Methods: A PD rat model was induced by 6-OHDA injections. Then the rats were transplanted intracerebroventricularly with HAE cells. Apomorphin-induced turns were used to assess the neurobehavioral deficit in rats. Immunofluorescence cytochemistry was used to track the survival of HAE cells. Tyrosinehydroxylase (TH) immunohistochemistry was used to determine the density of TH-positive cells in rat substantia nigra and the differentiation of HAE cells. High performance liquid chromatography (HPLC) was used to measure the levels of dopamine (DA) and its metabolites 3, 4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) in rat striatum. HVA levels in the cerebrospinal fluid of rats were also determined by HPLC. Results: The results showed that transplanted HAE cells can survive for at least 10 weeks and differentiate into TH-positive cells in PD rats. The grafts significantly ameliorated apomorphine-induced turns in PD rats. TH immunohistochemistry showed that HAE cells attenuated the loss of TH-positive cells in rat substantia nigra. In addition, HAE cells prevented the fall of DA and its metabolites DOPAC and HVA in PD rats. Increased HVA levels in the cerebrospinal fluid of PD rats were also observed. Conclusion: These results demonstrate that HAE cells have beneficial effect on 6-OHDA-induced PD rats, which may be due to the neurotrophic factors secrete by HAE cells.
引用
收藏
页码：1054 / 1059
页数：6
相关论文
共 33 条
  • [1] EXPRESSION OF HLA ANTIGENS, BETA-2-MICROGLOBULIN AND ENZYMES BY HUMAN AMNIOTIC EPITHELIAL-CELLS
    ADINOLFI, M
    AKLE, CA
    MCCOLL, I
    FENSOM, AH
    TANSLEY, L
    CONNOLLY, P
    HSI, BL
    FAULK, WP
    TRAVERS, P
    BODMER, WF
    [J]. NATURE, 1982, 295 (5847) : 325 - 327
  • [2] Nonviral glial cell-derived neurotrophic factor gene transfer enhances survival of cultured dopaminergic neurons and improves their function after transplantation in a rat model of Parkinson's disease
    Bauer, M
    Meyer, M
    Grimm, L
    Meitinger, T
    Zimmer, J
    Gasser, T
    Ueffing, M
    Widmer, HR
    [J]. HUMAN GENE THERAPY, 2000, 11 (11) : 1529 - 1541
  • [3] Comparative Characterization of Cultured Human Term Amnion Epithelial and Mesenchymal Stromal Cells for Application in Cell Therapy
    Bilic, Grozdana
    Zeisberger, Steffen M.
    Mallik, Ajit S.
    Zimmermann, Roland
    Zisch, Andreas H.
    [J]. CELL TRANSPLANTATION, 2008, 17 (08) : 955 - 968
  • [4] Partial recovery of dopaminergic pathway after graft of adult mesenchymal stem cells in a rat model of Parkinson's disease
    Bouchez, Gaelle
    Sensebe, Luc
    Vourc'h, Patrick
    Garreau, Lucette
    Bodard, Sylvie
    Rico, Angelique
    Guilloteau, Denis
    Charbord, Pierre
    Besnard, Jean-Claude
    Chalon, Sylvie
    [J]. NEUROCHEMISTRY INTERNATIONAL, 2008, 52 (07) : 1332 - 1342
  • [5] Modulation of brain-derived neurotrophic factor as a potential neuroprotective mechanism of action of omega-3 fatty acids in a parkinsonian animal model
    Bousquet, M.
    Gibrat, C.
    Saint-Pierre, M.
    Julien, C.
    Calon, F.
    Cicchetti, F.
    [J]. PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY, 2009, 33 (08) : 1401 - 1408
  • [6] Efficient derivation of functional dopaminergic neurons from human embryonic stem cells on a large scale
    Cho, Myung-Soo
    Hwang, Dong-Youn
    Kim, Dong-Wook
    [J]. NATURE PROTOCOLS, 2008, 3 (12) : 1888 - 1894
  • [7] Age-related changes in nigrostriatal dopaminergic function are accentuated in ± brain-derived neurotrophic factor mice
    Dluzen, DE
    McDermott, JL
    Anderson, LI
    Kucera, J
    Joyce, JN
    Osredkar, T
    Walro, JM
    [J]. NEUROSCIENCE, 2004, 128 (01) : 201 - 208
  • [8] Enosawa Shin, 2003, Nihon Rinsho, V61, P396
  • [9] Parkinson's disease - opportunities for novel therapeutics to reduce the problems of levodopa therapy
    Fox, Susan H.
    Chuang, Rosalind
    Brotchie, Jonathan M.
    [J]. SEROTONIN-DOPAMINE INTERACTION: EXPERIMENTAL EVIDENCE AND THERAPEUTIC RELEVANCE, 2008, 172 : 479 - 494
  • [10] Abnormal involuntary movements (AIMs) following pulsatile dopaminergic stimulation: Severe deterioration and morphological correlates following the loss of locus coeruleus neurons
    Fulceri, F.
    Biagioni, F.
    Ferrucci, M.
    Lazzeri, G.
    Bartalucci, A.
    Galli, V.
    Ruggieri, S.
    Paparelli, A.
    Fornai, F.
    [J]. BRAIN RESEARCH, 2007, 1135 (01) : 219 - 229
1234