Behavior of potato gametoclonal plants against the necrotic strain of potato Y potyvirus

被引:6
作者
Grammatikaki, G.
Avgelis, A.
Sonnino, A.
机构
[1] Inst Educ Technol, Sch Agr Technol, Dept Crop Sci, GR-71014 Iraklion, Greece
[2] Natl Agr Res Fdn, Plant Virus Lab, Iraklion, Greece
[3] Natl Agcy New Technologies Energy & Environm, Rome, Italy
关键词
Solanum tuberosum; gametoclonal variation; anther culture; potato virus Y; resistance; ANTHER CULTURE; SOLANUM-TUBEROSUM; VIRUS YN; RESISTANCE; TEMPERATURE; EXPRESSION; SYMPTOM; CLONES; GENES; WILD;
D O I
10.1134/S1021443707040115
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A total of 59 Solanum tuberosum androgenetic plants have been obtained through anther culture, 47 of which derived from a tetraploid clone, seven from a diploid hybrid, and five from an anther-derived clone. About two thirds of the anther-derived plants were dihaploids, a few were monohaploids (5.08%) or aneuploids (6.78%), whereas the tetraploid genotype generated about a third of tetraploids. Seven hundred twenty seven R-1 plants arisen from tubers of the androgenetic potatoes were mechanically inoculated with the necrotic strain of the potato Y potyvirus (PVYN) and grown in a glasshouse. Fifty days after inoculation, the presence of PVYN in R-1 plants was detected by DAS-ELISA (Double Autibody Sandwich). Only three plants (0.4%) of genotype H2-258 exhibited local necrotic symptoms (hypersensitivity reaction) suggesting the presence of the N (y) gene, and this extreme resistance is epistatic to hypersensitive resistance. The immunity (R (y)-gene) to PVYN was retained through anther culturing and present at all levels of ploidy. The pattern of segregation for immunity was differentiated according to the ploidy level of the anther-derived plants. This changed segregation pattern may be due to a loss of resistance during the culturing, when an endoreduplication has taken place or to the possible regeneration from Second-division restituted unreduced microspores. Anyway, this segregation pattern must be taken into account when gametoclones are used in genetic studies.
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页码:507 / 512
页数:6
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