In Vitro Study of DNA Interaction with Melamine and Its Related Compounds

被引:15
作者
Shen, Hao-Yu [1 ]
Liu, Yang-Qing [1 ]
Gao, Jing [1 ]
Zhen, Hui-Min [1 ]
Zhu, Ning [1 ]
Li, Jia [1 ]
机构
[1] Zhejiang Univ, Ningbo Inst Technol, Biol & Chem Engn Sch, Ningbo 315100, Zhejiang, Peoples R China
关键词
CALF THYMUS DNA; FLUORESCENCE PROBE; BINDING; COMPLEX; NETWORKS; SURFACE; ACIDS;
D O I
10.1089/dna.2010.1095
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In vitro studies on the interactions between native herring sperm DNA (HS-DNA) and melamine as well as its related compounds (MARCs), that is, ammeline, ammelide, and cyanuric acid, have been investigated by spectrophotometric, spectrofluorometric, melting temperature, and viscosimetric techniques. It was found that any of the MARCs might interact with HS-DNA by a groove mode of binding via hydrogen bonds. The interaction constants between any of the MARCs and HS-DNA were at 10(4)-10(5) L mol(-1), determined by both spectrophotometric and spectrofluorometric methods. The thermodynamic studies suggested that the interaction processes were exothermic favored (Delta H < 0) and entropy disfavored (Delta S < 0), which indicated that hydrogen bonds might be the main acting force in the binding of MARCs and DNA.
引用
收藏
页码:255 / 264
页数:10
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