Monitoring intracellular pH fluctuation with an excited-state intramolecular proton transfer-based ratiometric fluorescent sensor

被引：35

作者：

Feng, Bin ^{[1
,2
,3
]}

Zhu, Yingli ^{[1
,2
,3
]}

Wu, Jiaxin ^{[1
,2
,3
]}

Huang, Xueyan ^{[1
,2
,3
]}

Song, Rong ^{[1
,2
,3
]}

Huang, Liu ^{[1
,2
,3
]}

Feng, Xueping ^{[4
]}

Zeng, Wenbin ^{[1
,2
,3
]}

机构：

[1] Cent South Univ, Xiangya Sch Pharmaceut Sci, Changsha 410013, Peoples R China

[2] Cent South Univ, Mol Imaging Res Ctr, Changsha 410013, Peoples R China

[3] Cent South Univ, Hunan Key Lab Diagnost & Therapeut Drug Res Chron, Changsha 410013, Peoples R China

[4] Cent South Univ, Xiangya Hosp, Changsha 410013, Peoples R China

来源：

CHINESE CHEMICAL LETTERS | 2021年 / 32卷 / 10期

基金：

中国国家自然科学基金;

关键词：

Excited-state intramolecular proton transfer; Intracellular pH; Sulfonamide; Ratiometric sensor; Fluorescent imaging; METABOLIC-ACIDOSIS; LIVING CELLS; DELIVERY; DESIGN; COMMON; ESIPT; PROBE;

D O I：

10.1016/j.cclet.2021.03.074

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Intracellular pH is a key parameter related to various biological and pathological processes. In this study, a ratiometric pH fluorescent sensor ABTT was developed harnessing the amino-type excited-state intramolecular proton transfer (ESIPT) process. Relying on whether the ESIPT proceeds normally or not, ABTT exhibited the yellow fluorescence in acidic media, or cyan fluorescence in basic condition. According to the variation, ABTT behaved as a promising sensor which possessed fast and reversible response to pH change without interference from the biological substances, and exported a steady ratiometric signal (I-478/I-546). Moreover, due to the ESIPT effect, large Stokes shift and high quantum yield were also exhibited in ABTT. Furthermore, ABTT was applied for monitoring the pH changes in living cells and visualizing the pH fluctuations under oxidative stress successfully. These results elucidated great potential of ABTT in understanding pH-dependent physiological and pathological processes. (C) 2021 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.

引用

页码：3057 / 3060

页数：4

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