Cloning southern corn rust resistant gene RppK and its cognate gene AvrRppK from Puccinia polysora

被引：60

作者：

Chen, Gengshen ^{[1
]}

Zhang, Bao ^{[1
]}

Ding, Junqiang ^{[2
,3
]}

Wang, Hongze ^{[1
]}

Deng, Ce ^{[2
]}

Wang, Jiali ^{[1
]}

Yang, Qianhui ^{[1
]}

Pi, Qianyu ^{[1
]}

Zhang, Ruyang ^{[4
]}

Zhai, Haoyu ^{[1
]}

Dong, Junfei ^{[1
]}

Huang, Junshi ^{[1
]}

Hou, Jiabao ^{[1
]}

Wu, Junhua ^{[1
]}

Que, Jiamin ^{[1
]}

Zhang, Fan ^{[1
]}

Li, Wenqiang ^{[1
]}

Min, Haoxuan ^{[1
]}

Tabor, Girma ^{[5
]}

Li, Bailin ^{[5
]}

Liu, Xiangguo ^{[6
]}

Zhao, Jiuran ^{[4
]}

Yan, Jianbing ^{[1
,7
]}

Lai, Zhibing ^{[1
,7
]}

机构：

[1] Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Hubei, Peoples R China

[2] Henan Agr Univ, Coll Agron, Zhengzhou 450002, Henan, Peoples R China

[3] Shennong Lab, Zhengzhou 450002, Henan, Peoples R China

[4] Beijing Acad Agr & Forestry Sci BAAFS, Maize Res Ctr, Beijing 100097, Peoples R China

[5] Corteva Agrisci, Johnston, IA 50131 USA

[6] Jilin Acad Agr Sci, Inst Agr Biotechnol, Changchun 130033, Jilin, Peoples R China

[7] Hubei Hongshan Lab, Wuhan 430070, Hubei, Peoples R China

来源：

NATURE COMMUNICATIONS | 2022年 / 13卷 / 01期

基金：

中国国家自然科学基金;

关键词：

CONFERS QUANTITATIVE RESISTANCE; SUGARCANE-MOSAIC-VIRUS; WHEAT-STEM RUST; DISEASE RESISTANCE; STALK ROT; MAIZE; EFFECTORS; DISSECTION; VIRULENCE; IMMUNITY;

D O I：

10.1038/s41467-022-32026-4

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Southern corn rust (SCR) caused by Puccinia polysora is a major maize disease that can result in major yield loss. Here, the authors report the expression of a CC-NB-LRR type of R gene RppK results in SCR resistance in susceptible maize lines and it can recognize the effector AvrRppK produced by P. polysora. Broad-spectrum resistance has great values for crop breeding. However, its mechanisms are largely unknown. Here, we report the cloning of a maize NLR gene, RppK, for resistance against southern corn rust (SCR) and its cognate Avr gene, AvrRppK, from Puccinia polysora (the causal pathogen of SCR). The AvrRppK gene has no sequence variation in all examined isolates. It has high expression level during infection and can suppress pattern-triggered immunity (PTI). Further, the introgression of RppK into maize inbred lines and hybrids enhances resistance against multiple isolates of P. polysora, thereby increasing yield in the presence of SCR. Together, we show that RppK is involved in resistance against multiple P. polysora isolates and it can recognize AvrRppK, which is broadly distributed and conserved in P. polysora isolates.

引用

页数：11

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