Analysis of HIV type 1 protease and reverse transcriptase sequences from Venezuela for drug resistance-associated mutations and subtype classification:: A UNAIDS study

被引:30
作者
Delgado, E
León-Ponte, M
Villahermosa, ML
Cuevas, MT
Deibis, L
Echeverría, G
Thomson, MM
Pérez-Alvarez, L
Osmanov, S
Nájera, R
机构
[1] Inst Salud Carlos III, Ctr Nacl Biol Fundamental, Madrid 28220, Spain
[2] Cent Univ Venezuela, Caracas, Venezuela
[3] UNAIDS, Geneva, Switzerland
关键词
D O I
10.1089/088922201750237022
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
We report the first study on prevalence of antiretroviral drug-associated resistance mutations in Venezuela, Protease and reverse transcriptase (RT) coding regions were analyzed in DNA samples obtained from 100 HIV-1-infected individuals. Primary resistance mutations to RT inhibitors were identified in 26% of patients treated with these drugs, Transmission of HIV-1-resistant strains was detected in a drug-naive patient (3%), Primary resistance mutations to protease inhibitors (PIs) were present in 9% of the 44 PI-treated patients and in 1 PI-naive individual. Phylogenetic analysis of these samples has resulted in the most extensive survey, to date, of HIV-1 genetic forms circulating in Venezuela, Ninety-nine samples clustered with subtype B, and 1 individual harbored the first B/F recombinant virus reported in Venezuela, with protease clustering with subtype F and RT with subtype B, In addition, this isolate had a new insertion (Glu-34 duplication) in the protease gene.
引用
收藏
页码:753 / 758
页数:6
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